Sadiq M F, Khabour O F
Department of Biological Sciences, Yarmouk University, Irbid, Jordan.
Mutat Res. 1998 Feb 2;397(2):279-85. doi: 10.1016/s0027-5107(97)00226-1.
The mutagenicity of bleomycin was studied in the different stages of spermatogenesis in Drosophila melanogaster. Following the injection of 2 microliters of 0.1 micrograms/ml of the chemical into young wild-type males, complete and mosaic sex-linked recessive lethals were scored by the Muller-5 method in five successive broods, mainly representing the different stages of spermatogenesis. The delayed mutagenic effect of the chemical was measured by the proportion of mosaic progeny produced. The results showed that bleomycin significantly increased the proportions of both complete and mosaic lethals in the broods representing the meiotic and pre-meiotic stages, but did not show any significant increase in these proportions in the broods representing the sperms and spermatids. The sizes of the mutated areas in the F1 gonads represented by the proportions of lethal-bearing females in F2 mosaic cultures were small, indicating that the genetic instabilities induced by bleomycin were transformed into actual mutations in later zygotic divisions. The significant divisions. The significant production of mosaic progeny in the F4 generation of the treated males showed that the mosaic F1 females produced by bleomycin were able to produce further mosaic progeny and suggested that bleomycin-induced instabilities can be transmitted as such for many future generations.
在黑腹果蝇精子发生的不同阶段研究了博来霉素的致突变性。将2微升0.1微克/毫升的该化学物质注射到年轻的野生型雄性果蝇体内后，通过Muller-5方法在连续五个批次中对完全和嵌合的性连锁隐性致死突变进行评分，这些批次主要代表精子发生的不同阶段。通过产生的嵌合后代比例来测量该化学物质的延迟诱变效应。结果表明，博来霉素在代表减数分裂和减数分裂前期阶段的批次中显著增加了完全致死突变和嵌合致死突变的比例，但在代表精子和精细胞的批次中这些比例没有显著增加。以F2嵌合培养物中携带致死突变的雌性比例表示的F1性腺中突变区域的大小较小，这表明博来霉素诱导的遗传不稳定性在随后的合子分裂中转化为实际突变。处理过的雄性果蝇F4代中显著产生嵌合后代，这表明博来霉素产生的嵌合F1雌性能够产生更多的嵌合后代，并表明博来霉素诱导的不稳定性能够原样传递给许多后代。
