Huang C Y, Patel B K, Mah R A, Baresi L
Department of Environmental Health Sciences, School of Public Health, University of California, Los Angeles 90095-1772, USA.
Int J Syst Bacteriol. 1998 Jan;48 Pt 1:91-7. doi: 10.1099/00207713-48-1-91.
An anaerobic, extremely thermophilic, xylanolytic, non-spore-forming bacterium was isolated from a sediment sample taken from Owens Lake, California, and designated strain OLT (T = type strain). Strain OLT had a Gramnegative reaction and occurred as short rods which sometimes formed long chains containing a few coccoid cells. It grew at 50-80 degrees C, with an optimum at 75 degrees C. The pH range for growth was 5.5-9.0 with an optimum at about pH 7.5. When grown on glucose at optimal conditions, its doubling time was 7.3 h. In addition to glucose, the isolate utilized sucrose, xylose, fructose, ribose, xylan, starch, pectin and cellulose. Yeast extract stimulated growth on carbohydrates but was not obligately required. The end products from glucose fermentation were lactate, acetate, ethanol, H2 and CO2. The G + C content of strain OLT was 36.6 mol%. The 16S rDNA sequence analysis indicated that strain OLT was a member of the subdivision containing Gram-positive bacteria with DNA G + C content of less than 55 mol% and clustered with members of the genus Caldicellulosiruptor. Because strain OLT is phylogenetically and phenotypically different from other members of this genus, it is proposed to designate this isolate Caldicellulosiruptor owensensis sp. nov. Strain OLT is the type strain (= ATCC 700167T).
从加利福尼亚州欧文斯湖采集的沉积物样本中分离出一种厌氧、嗜热、能分解木聚糖且不形成芽孢的细菌，命名为OLT菌株（T = 模式菌株）。OLT菌株呈革兰氏阴性反应，为短杆菌，有时形成长链，其中含有一些球状细胞。它在50 - 80℃生长，最适生长温度为75℃。生长的pH范围是5.5 - 9.0，最适pH约为7.5。在最佳条件下于葡萄糖上生长时，其倍增时间为7.3小时。除葡萄糖外，该分离株还利用蔗糖、木糖、果糖、核糖、木聚糖、淀粉、果胶和纤维素。酵母提取物可刺激碳水化合物上的生长，但并非必需。葡萄糖发酵的终产物为乳酸、乙酸、乙醇、H₂和CO₂。OLT菌株的G + C含量为36.6 mol%。16S rDNA序列分析表明，OLT菌株是DNA G + C含量低于55 mol%的革兰氏阳性细菌分类中的一员，与嗜热栖热菌属的成员聚类。由于OLT菌株在系统发育和表型上与该属的其他成员不同，因此建议将此分离株命名为欧文斯嗜热栖热菌（Caldicellulosiruptor owensensis）新种。OLT菌株是模式菌株（= ATCC 700167T）。
