Slobodkin A, Reysenbach A L, Strutz N, Dreier M, Wiegel J
Department of Microbiology, University of Georgia, Athens 30602-2605, USA.
Int J Syst Bacteriol. 1997 Apr;47(2):541-7. doi: 10.1099/00207713-47-2-541.
A strain of a thermophilic, anaerobic, dissimilatory, Fe(III)-reducing bacterium, Thermoterrabacterium ferrireducens gen. nov., sp. nov. (type strain JW/AS-Y7T; DSM 11255), was isolated from hot springs in Yellowstone National Park and New Zealand. The gram-positive-staining cells occurred singly or in pairs as straight to slightly curved rods, 0.3 to 0.4 by 1.6 to 2.7 microns, with rounded ends and exhibited a tumbling motility. Spores were not observed. The temperature range for growth was 50 to 74 degrees C with an optimum at 65 degrees C. The pH range for growth at 65 degrees C was from 5.5 to 7.6, with an optimum at 6.0 to 6.2. The organism coupled the oxidation of glycerol to reduction of amorphous Fe(III) oxide or Fe(III) citrate as an electron acceptor. In the presence as well as in the absence of Fe(III) and in the presence of CO2, glycerol was metabolized by incomplete oxidation to acetate as the only organic metabolic product; no H2 was produced during growth. The organism utilized glycerol, lactate, 1,2-propanediol, glycerate, pyruvate, glucose, fructose, mannose, and yeast extract as substrates. In the presence of Fe(III) the bacterium utilized molecular hydrogen. The organism reduced 9,10-anthraquinone-2,6-disulfonic acid, fumarate (to succinate), and thiosulfate (to elemental sulfur) but did not reduce MnO2, nitrate, sulfate, sulfite, or elemental sulfur. The G + C content of the DNA was 41 mol% (as determined by high-performance liquid chromatography). The 16S ribosomal DNA sequence analysis placed the isolated strain as a member of a new genus within the gram-type-positive Bacillus-Clostridium subphylum.
从黄石国家公园和新西兰的温泉中分离出了一株嗜热、厌氧、异化型铁（III）还原细菌——铁还原嗜热地杆菌（Thermoterrabacterium ferrireducens），新属，新种（模式菌株JW/AS-Y7T；DSM 11255）。革兰氏阳性染色细胞单个或成对出现，为直杆状至微弯杆状，大小为0.3至0.4×1.6至2.7微米，末端圆钝，具有翻滚运动性。未观察到孢子。生长温度范围为50至74℃，最适温度为65℃。在65℃下生长的pH范围为5.5至7.6，最适pH为6.0至6.2。该生物体将甘油的氧化与无定形氧化铁或柠檬酸铁（III）的还原偶联，作为电子受体。在有或没有铁（III）存在以及有二氧化碳存在的情况下，甘油通过不完全氧化代谢为乙酸盐，作为唯一的有机代谢产物；生长过程中不产生氢气。该生物体利用甘油、乳酸、1,2 - 丙二醇、甘油酸、丙酮酸、葡萄糖、果糖、甘露糖和酵母提取物作为底物。在有铁（III）存在的情况下，该细菌利用分子氢。该生物体可还原9,10 - 蒽醌 - 2,6 - 二磺酸、富马酸盐（还原为琥珀酸盐）和硫代硫酸盐（还原为元素硫），但不还原二氧化锰、硝酸盐、硫酸盐、亚硫酸盐或元素硫。DNA的G + C含量为41 mol%（通过高效液相色谱法测定）。16S核糖体DNA序列分析表明，分离出的菌株属于革兰氏阳性芽孢杆菌 - 梭菌亚门中的一个新属。
