Martí E, Ferrer I, Ballabriga J, Blasi J
Departament de Biologia Cellular i Anatomia Patològica, Universitat de Barcelona, Spain.
Acta Neuropathol. 1998 Mar;95(3):254-60. doi: 10.1007/s004010050795.
SNAP-25 (a synaptosomal-associated protein of 25 kDa) has been shown to be involved both in synaptic vesicle exocytosis and in axonal outgrowth. In the present study, we investigated the changes in SNAP-25 immunoreactivity in the hippocampus of the Mongolian gerbil (Meriones unguiculatus) at different time points after transient forebrain ischemia insult. In parallel, immunostaining for GAP-43, a protein involved in axonal outgrowth, and for syntaxin-1 (stx1A and stx1B), another protein implicated in neurotransmitter release, was also analyzed. The animals were subjected to 2.5 or 5 min of transient forebrain ischemia through bilateral common carotid occlusion, and examined at different intervals after ischemia. SNAP-25 immunoreactivity was increased in the mossy fiber layer as early as 2 days after 5 min of ischemia. Increased SNAP-25 immunoreactivity in mossy fibers was also detected at days 4 and 7 after ischemia. On day 15, SNAP-25 staining was similar to that observed in control non-ischemic animals. In contrast, no changes in GAP-43 and syntaxin-1 immunoreactivity were observed in the mossy fiber layer following 5 min of ischemia. No modifications in SNAP-25, syntaxin-1 or GAP-43 immunoreactivity were observed following 2.5 min of ischemia, the longest period for which no neuronal damage is observed. These results provide evidence of a specific involvement of SNAP-25 in the reactive changes associated with transient forebrain ischemia.
SNAP-25（一种25 kDa的突触体相关蛋白）已被证明与突触小泡胞吐作用和轴突生长均有关。在本研究中，我们调查了蒙古沙鼠（长爪沙鼠）前脑短暂缺血损伤后不同时间点海马中SNAP-25免疫反应性的变化。同时，还分析了参与轴突生长的蛋白GAP-43以及另一种与神经递质释放有关的蛋白 syntaxin-1（stx1A和stx1B）的免疫染色情况。通过双侧颈总动脉闭塞使动物经历2.5或5分钟的前脑短暂缺血，并在缺血后的不同时间间隔进行检查。缺血5分钟后仅2天，苔藓纤维层中的SNAP-25免疫反应性就增加了。在缺血后的第4天和第7天，苔藓纤维中也检测到SNAP-25免疫反应性增加。在第15天，SNAP-25染色与未缺血对照动物中观察到的相似。相比之下，缺血5分钟后苔藓纤维层中未观察到GAP-43和syntaxin-1免疫反应性的变化。缺血2.5分钟（未观察到神经元损伤的最长时间）后，未观察到SNAP-25、syntaxin-1或GAP-43免疫反应性的改变。这些结果提供了SNAP-25特异性参与与前脑短暂缺血相关的反应性变化的证据。
