Knepper P A, Mayanil C S, Goossens W, McLone D C, Hayes E
Laboratory for Oculo-Cerebrospinal Investigation, Children's Memorial Medical Center, Chicago, Illinois 60614, USA.
In Vitro Cell Dev Biol Anim. 1998 Feb;34(2):170-3. doi: 10.1007/s11626-998-0101-2.
Three sources of fetal bovine serum (FBS) were fractionated by ammonium sulfate precipitation and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to Immobilon-P membranes, immunoblotted with a panel of transcription factor antibodies, and detected by enhanced chemiluminescence. Nine transcription factors were detected--ATF-2, SRE-ZBP, GATA-2, TFIID, Ets-1/Ets-2, E2F-1, Oct-2, p53, and AP-2; four transcription factors were not detected--Myo D, CREB, Sp2, and Wilms' tumor. The results indicated the presence of varying amounts of several transcription factors in three commercial sources and may represent heretofore unrecognized factors influencing cell culture.
采用硫酸铵沉淀法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对三种胎牛血清（FBS）来源进行分级分离，转移至Immobilon-P膜上，用一组转录因子抗体进行免疫印迹，并通过增强化学发光法进行检测。检测到9种转录因子——ATF-2、SRE-ZBP、GATA-2、TFIID、Ets-1/Ets-2、E2F-1、Oct-2、p53和AP-2；未检测到4种转录因子——Myo D、CREB、Sp2和威尔姆斯瘤。结果表明三种商业来源中存在不同数量的几种转录因子，可能代表了迄今为止未被认识到的影响细胞培养的因子。
