Frontier Lifeline Pvt. Ltd., TICEL Biopark, Taramani, Chennai 600113, India.
Stem Cells Int. 2011;2011:504723. doi: 10.4061/2011/504723. Epub 2011 Apr 3.
Cellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to regenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow, adipose tissue, and so forth, bring forth the need for their in vitro culture/expansion. However, the extensive usage of supplements containing xenogeneic components in the expansion-media might pose a risk to the post-transplantation safety of patients. This warrants the necessity to identify and develop chemically defined or "humanized" supplements which would make in vitro cultured/processed cells relatively safer for transplantation in regenerative medicine. In this paper, we outline the various caveats associated with conventionally used supplements of xenogenic origin and also portray the possible alternatives/additives which could one day herald the dawn of a new era in the translation of in vitro cultured cells to therapeutic interventions.
细胞治疗正在达到一个高峰，人们越来越了解人类间充质干细胞（hMSCs）在体内再生受损组织的潜力。目前在骨髓、脂肪组织等已确定的来源中，这些 hMSCs 的数量有限，因此需要进行体外培养/扩增。然而，在扩增培养基中广泛使用含有异种成分的补充剂可能会对患者移植后的安全性构成风险。这就需要我们识别和开发化学定义或“人源化”的补充剂，以使体外培养/加工的细胞在再生医学中的移植相对更安全。在本文中，我们概述了传统使用的异种来源补充剂所涉及的各种注意事项，并描述了可能的替代物/添加剂，这些替代物/添加剂可能有一天会开创一个新的时代，将体外培养的细胞转化为治疗干预措施。
