A组链球菌的菌毛类型具有M蛋白基因（emm）序列类型特异性。
Group A streptococcal Vir types are M-protein gene (emm) sequence type specific.
作者信息
Gardiner D L, Goodfellow A M, Martin D R, Sriprakash K S
机构信息
Menzies School of Health Research, Darwin, Australia.
出版信息
J Clin Microbiol. 1998 Apr;36(4):902-7. doi: 10.1128/JCM.36.4.902-907.1998.
Abstract
The M-protein genes (emm genes) of 103 separate impetiginous Streptococcus pyogenes isolates were sequenced and the sequence types were compared to the types obtained by Vir typing. Vir typing is based on restriction fragment length polymorphism (RFLP) analysis of a 4- to 7-kb pathogenicity island encoding emm and other virulence genes. By using both HaeIII and HinfI to generate RFLP profiles, complete concordance between Vir type and emm sequence type was found. Comparison of the emm sequences with those in GenBank revealed new sequence types sharing less than 90% identity with known types. Diversity in the emm sequence was generated by corrected frameshift mutations, point mutations, and small in-frame mutations.
摘要
对103株不同的脓疱性化脓性链球菌分离株的M蛋白基因（emm基因）进行了测序，并将序列类型与通过Vir分型获得的类型进行了比较。Vir分型基于对一个4至7kb的致病岛进行限制性片段长度多态性（RFLP）分析，该致病岛编码emm和其他毒力基因。通过使用HaeIII和HinfI两者来生成RFLP图谱，发现Vir型与emm序列型完全一致。将emm序列与GenBank中的序列进行比较，发现了与已知类型同一性低于90%的新序列类型。emm序列的多样性是由校正后的移码突变、点突变和小的框内突变产生的。
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