用于诊断类鼻疽的聚合酶链反应评估。
Evaluation of PCR for diagnosis of melioidosis.
作者信息
Haase A, Brennan M, Barrett S, Wood Y, Huffam S, O'Brien D, Currie B
机构信息
Royal Darwin Hospital, Tiwi, Northern Territory, Australia.
出版信息
J Clin Microbiol. 1998 Apr;36(4):1039-41. doi: 10.1128/JCM.36.4.1039-1041.1998.
Abstract
Previously published PCR-based diagnostic tests for melioidosis were evaluated for clinical usefulness. A Burkholderia pseudomallei 16S rRNA-derived primer set had a sensitivity approaching 100% for clinical samples from 22 culture-confirmed cases of melioidosis and enabled diagnosis of 3 culture-negative cases. However, samples from 10 of 30 inpatients from Royal Darwin Hospital with other diagnoses were positive by PCR, giving a specificity of 67% and a positive predictive value of only 70%. Although there are a number of intriguing possible explanations for our results, concerns of inappropriate therapy resulting from a positive result by PCR have led us to forgo the advantage of rapid PCR diagnosis for melioidosis until a better system is validated.
摘要
我们对之前发表的基于聚合酶链反应（PCR）的类鼻疽诊断测试的临床实用性进行了评估。一套源自伯克霍尔德菌假鼻疽16S核糖体RNA的引物对22例经培养确诊的类鼻疽临床样本的敏感性接近100%，并能够诊断出3例培养阴性的病例。然而，来自达尔文皇家医院30例患有其他疾病的住院患者中的10例样本经PCR检测呈阳性，特异性为67%，阳性预测值仅为70%。尽管对于我们的结果有许多有趣的可能解释，但由于PCR阳性结果导致不适当治疗的担忧，使我们在更好的系统得到验证之前，放弃了PCR快速诊断类鼻疽的优势。
相似文献
1
Evaluation of PCR for diagnosis of melioidosis.用于诊断类鼻疽的聚合酶链反应评估。
J Clin Microbiol. 1998 Apr;36(4):1039-41. doi: 10.1128/JCM.36.4.1039-1041.1998.
2
Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis.16S rRNA实时荧光定量PCR检测法诊断类鼻疽准确性的前瞻性临床评估
Am J Trop Med Hyg. 2007 Nov;77(5):814-7.
3
Comparison of three PCR primer sets for diagnosis of septicemic melioidosis.用于诊断败血性类鼻疽的三种聚合酶链反应引物组的比较
Acta Trop. 2000 Feb 5;74(2-3):247-51. doi: 10.1016/s0001-706x(99)00077-7.
4
Clinico-microbiological description and evaluation of rapid lateral flow immunoassay and PCR for detection of Burkholderia pseudomallei from patients hospitalized with sepsis and pneumonia: A twenty-one months study from Odisha, India.从印度奥里萨邦一项为期 21 个月的研究：对快速侧向流动免疫测定和 PCR 检测败血症和肺炎住院患者中类鼻疽伯克霍尔德菌的临床微生物学描述和评估。
Acta Trop. 2021 Sep;221:105994. doi: 10.1016/j.actatropica.2021.105994. Epub 2021 Jun 9.
5
6
Laboratory diagnosis of melioidosis: past, present and future.类鼻疽的实验室诊断：过去、现在与未来
Exp Biol Med (Maywood). 2015 Jun;240(6):742-51. doi: 10.1177/1535370215583801. Epub 2015 Apr 22.
7
Improved detection of Burkholderia pseudomallei from non-blood clinical specimens using enrichment culture and PCR: narrowing diagnostic gap in resource-constrained settings.使用富集培养和聚合酶链反应（PCR）改进从非血液临床标本中检测类鼻疽伯克霍尔德菌：缩小资源受限环境中的诊断差距
Trop Med Int Health. 2017 Jul;22(7):866-870. doi: 10.1111/tmi.12894. Epub 2017 Jun 8.
8
Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis.用于诊断类鼻疽的III型分泌系统实时聚合酶链反应检测法的临床评估
J Clin Microbiol. 2006 Aug;44(8):3028-30. doi: 10.1128/JCM.00913-06.
9
Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes.使用荧光杂交探针通过实时聚合酶链反应检测法快速初步鉴定类鼻疽伯克霍尔德菌。
Mol Cell Probes. 2005 Feb;19(1):9-20. doi: 10.1016/j.mcp.2004.08.001.
10
Performance evaluation of Active Melioidosis Detect-Lateral Flow Assay (AMD-LFA) for diagnosis of melioidosis in endemic settings with limited resources.在资源有限的地方性流行环境中，用于诊断类鼻疽病的主动类鼻疽病检测-侧向流动检测法（AMD-LFA）的性能评估。
PLoS One. 2018 Mar 26;13(3):e0194595. doi: 10.1371/journal.pone.0194595. eCollection 2018.
引用本文的文献
1
Pyrexia of Unknown Origin in a Young Male: Unmasking Melioidosis in a Tuberculosis-Endemic Setting.一名年轻男性的不明原因发热：在结核病流行地区揭示类鼻疽病
Cureus. 2024 Jul 7;16(7):e64024. doi: 10.7759/cureus.64024. eCollection 2024 Jul.
2
Adapting Microarray Gene Expression Signatures for Early Melioidosis Diagnosis.调整微阵列基因表达特征用于类鼻疽早期诊断
J Clin Microbiol. 2020 Jun 24;58(7). doi: 10.1128/JCM.01906-19.
3
Melioidosis: An Australian Perspective.类鼻疽：澳大利亚视角
Trop Med Infect Dis. 2018 Mar 1;3(1):27. doi: 10.3390/tropicalmed3010027.
4
5
Development of a diagnostic system for Burkholderia pseudomallei infections.建立一种用于诊断伯克霍尔德氏菌感染的诊断系统。
World J Microbiol Biotechnol. 2012 Jul;28(7):2465-71. doi: 10.1007/s11274-012-1053-y. Epub 2012 May 23.
6
Primary ocular melioidosis due to a single genotype of Burkholderia pseudomallei in two cats from Arnhem Land in the Northern Territory of Australia.澳大利亚北领地阿纳姆地的两只猫因单一基因型类鼻疽杆菌感染引发原发性眼鼻疽病。
J Feline Med Surg. 2009 Oct;11(10):856-63. doi: 10.1016/j.jfms.2009.02.009. Epub 2009 May 9.
7
Development of real-time PCR assays and evaluation of their potential use for rapid detection of Burkholderia pseudomallei in clinical blood specimens.实时聚合酶链反应检测方法的开发及其在临床血液标本中快速检测伯克霍尔德菌的潜在用途评估。
J Clin Microbiol. 2007 Sep;45(9):2894-901. doi: 10.1128/JCM.00291-07. Epub 2007 Jul 18.
8
Use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of Burkholderia pseudomallei infection in patients with community-acquired septicemia.使用基于蛋白质特异性单克隆抗体的乳胶凝集试验对社区获得性败血症患者进行类鼻疽伯克霍尔德菌感染的快速诊断。
Clin Vaccine Immunol. 2007 Jun;14(6):811-2. doi: 10.1128/CVI.00011-07. Epub 2007 Apr 11.
9
Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis.用于诊断类鼻疽的III型分泌系统实时聚合酶链反应检测法的临床评估
J Clin Microbiol. 2006 Aug;44(8):3028-30. doi: 10.1128/JCM.00913-06.
10
Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei.针对类鼻疽伯克霍尔德菌III型分泌系统的实时荧光定量PCR检测方法的开发与评估
J Clin Microbiol. 2006 Jan;44(1):85-90. doi: 10.1128/JCM.44.1.85-90.2006.
本文引用的文献
1
2
Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains.类鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌样菌株的特性分析。
Epidemiol Infect. 1997 Apr;118(2):137-48. doi: 10.1017/s095026889600739x.
3
Detection of Burkholderia pseudomallei DNA in patients with septicemic melioidosis.败血症型类鼻疽患者中伯克霍尔德菌属假鼻疽杆菌DNA的检测
J Clin Microbiol. 1996 Mar;34(3):609-14. doi: 10.1128/jcm.34.3.609-614.1996.
4
Intracellular survival of Burkholderia pseudomallei.类鼻疽伯克霍尔德菌的细胞内存活
Infect Immun. 1996 Mar;64(3):782-90. doi: 10.1128/iai.64.3.782-790.1996.
5
Medicine in tropical Australia.澳大利亚热带地区的医学
Med J Aust. 1993 May 3;158(9):609, 612-5. doi: 10.5694/j.1326-5377.1993.tb137630.x.
6
Relapse in melioidosis: incidence and risk factors.类鼻疽病的复发：发病率及危险因素
J Infect Dis. 1993 Nov;168(5):1181-5.
7
Effective amplification of long targets from cloned inserts and human genomic DNA.从克隆插入片段和人类基因组DNA中有效扩增长片段靶标。
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695-9. doi: 10.1073/pnas.91.12.5695.
8
The 1990-1991 outbreak of melioidosis in the Northern Territory of Australia: clinical aspects.1990 - 1991年澳大利亚北领地类鼻疽病疫情：临床情况
Southeast Asian J Trop Med Public Health. 1993 Sep;24(3):436-43.
9
Ceftazidime vs. amoxicillin/clavulanate in the treatment of severe melioidosis.头孢他啶与阿莫西林/克拉维酸治疗严重类鼻疽病的对比研究
Clin Infect Dis. 1994 Nov;19(5):846-53. doi: 10.1093/clinids/19.5.846.
10
Latex agglutination test for identification of Pseudomonas pseudomallei.用于鉴定类鼻疽假单胞菌的乳胶凝集试验。
J Clin Pathol. 1993 Apr;46(4):374-5. doi: 10.1136/jcp.46.4.374.
Zotero 插件