一种基于任意引物PCR的优化且高鉴别力方法在耐甲氧西林金黄色葡萄球菌医院感染流行病学分析中的应用
Application of an optimized and highly discriminatory method based on arbitrarily primed PCR for epidemiologic analysis of methicillin-resistant Staphylococcus aureus nosocomial infections.
作者信息
Olmos A, Camarena J J, Nogueira J M, Navarro J C, Risen J, Sánchez R
机构信息
Department of Microbiology, Hospital Dr. Peset, Valencia, Spain.
出版信息
J Clin Microbiol. 1998 Apr;36(4):1128-34. doi: 10.1128/JCM.36.4.1128-1134.1998.
Abstract
The optimization of an arbitrarily primed PCR method for typing 96 methicillin-resistant Staphylococcus aureus (MRSA) isolates was compared with pulsed-field gel electrophoresis. Identical results in the differentiation of MRSA clones and identification of the main cluster that included 82 strains (88% of patients) were obtained by both techniques.
摘要
将用于对96株耐甲氧西林金黄色葡萄球菌（MRSA）分离株进行分型的任意引物PCR方法的优化与脉冲场凝胶电泳进行了比较。两种技术在区分MRSA克隆和鉴定包含82株菌株（占患者的88%）的主要聚类方面获得了相同的结果。
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