肺炎链球菌在多塞特卵培养基上于室温下的长期存活情况。
Long-term survival of Streptococcus pneumoniae at room temperature on Dorset egg medium.
作者信息
Wasas A D, Huebner R E, De Blanche M, Klugman K P
机构信息
MRC/SAIMR/WITS Pneumococcal Diseases Research Unit, South African Institute for Medical Research, Hillbrow, Johannesburg.
出版信息
J Clin Microbiol. 1998 Apr;36(4):1139-40. doi: 10.1128/JCM.36.4.1139-1140.1998.
Abstract
Forty-five isolates of Streptococcus pneumoniae were inoculated on Dorset egg and supplemented Columbia agar base media, incubated overnight at 37 degrees C, and then kept at room temperature (RT; 21 degrees C) or 4 degrees C. Long-term viability was best at RT for both media, with all isolates remaining viable on Dorset egg medium for 44 days; viability was 90 and 57% on Columbia agar base medium after 7 and 30 days. We recommend the use of Dorset egg medium for the maintenance of pneumococci at RT.
摘要
将45株肺炎链球菌接种于多西氏鸡蛋培养基和添加了添加剂的哥伦比亚琼脂基础培养基上，在37℃下过夜培养，然后置于室温（RT；21℃）或4℃保存。两种培养基在室温下长期存活率最佳，所有菌株在多西氏鸡蛋培养基上44天仍存活；在哥伦比亚琼脂基础培养基上，7天和30天后存活率分别为90%和57%。我们建议使用多西氏鸡蛋培养基在室温下保存肺炎球菌。
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