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PLoS One. 2013 Aug 7;8(8):e72353. doi: 10.1371/journal.pone.0072353. Print 2013.
8
Quantitative validation of media for transportation and storage of Streptococcus pneumoniae.用于肺炎链球菌运输和储存的培养基的定量验证
J Clin Microbiol. 2003 Dec;41(12):5551-6. doi: 10.1128/JCM.41.12.5551-5556.2003.
9
Use of Dorset egg medium for maintenance and transport of Neisseria meningitidis and Haemophilus influenzae type b.使用多塞特卵培养基来保存和运输脑膜炎奈瑟菌和b型流感嗜血杆菌。
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本文引用的文献

1
Pneumococcal resistance to antibiotics.肺炎球菌对抗生素的耐药性。
Clin Microbiol Rev. 1990 Apr;3(2):171-96. doi: 10.1128/CMR.3.2.171.
2
The magnitude of mortality from acute respiratory infections in children under 5 years in developing countries.发展中国家5岁以下儿童急性呼吸道感染的死亡率规模。
World Health Stat Q. 1992;45(2-3):180-91.

肺炎链球菌在多塞特卵培养基上于室温下的长期存活情况。

Long-term survival of Streptococcus pneumoniae at room temperature on Dorset egg medium.

作者信息

Wasas A D, Huebner R E, De Blanche M, Klugman K P

机构信息

MRC/SAIMR/WITS Pneumococcal Diseases Research Unit, South African Institute for Medical Research, Hillbrow, Johannesburg.

出版信息

J Clin Microbiol. 1998 Apr;36(4):1139-40. doi: 10.1128/JCM.36.4.1139-1140.1998.

DOI:10.1128/JCM.36.4.1139-1140.1998
PMID:9542956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC104708/
Abstract

Forty-five isolates of Streptococcus pneumoniae were inoculated on Dorset egg and supplemented Columbia agar base media, incubated overnight at 37 degrees C, and then kept at room temperature (RT; 21 degrees C) or 4 degrees C. Long-term viability was best at RT for both media, with all isolates remaining viable on Dorset egg medium for 44 days; viability was 90 and 57% on Columbia agar base medium after 7 and 30 days. We recommend the use of Dorset egg medium for the maintenance of pneumococci at RT.

摘要

将45株肺炎链球菌接种于多西氏鸡蛋培养基和添加了添加剂的哥伦比亚琼脂基础培养基上，在37℃下过夜培养，然后置于室温（RT；21℃）或4℃保存。两种培养基在室温下长期存活率最佳，所有菌株在多西氏鸡蛋培养基上44天仍存活；在哥伦比亚琼脂基础培养基上，7天和30天后存活率分别为90%和57%。我们建议使用多西氏鸡蛋培养基在室温下保存肺炎球菌。