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两种补充培养基用于菌株的长期室温保存的评估。

Evaluation of Two Supplemented Culture Media for Long-Term, Room-Temperature Preservation of Strains.

机构信息

Departamento de Microbiología y Parasitología Clínicas, Facultad de Medicina, Universidad de Los Andes, Mérida 5101, Venezuela.

Laboratorio de Microbiología Molecular, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida 5101, Venezuela.

出版信息

Biomed Res Int. 2017;2017:1218798. doi: 10.1155/2017/1218798. Epub 2017 Nov 22.

DOI:10.1155/2017/1218798
PMID:29359142
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5735590/
Abstract

OBJECTIVE

To produce two supplemented agar types in order to store pneumococci for several months at room temperature.

METHODS

Todd-Hewitt/Hemoglobin/Yeast/Charcoal/Agar (TH-HYC) and Todd-Hewitt/Skim-Milk/Yeast/Charcoal/Agar (TH-SYC) were used to prepare two supplemented agar types. Nineteen pneumococci isolated from patients or asymptomatic carriers displaying diverse serotypes and multilocus sequence types (MLST) were subcultured and stored onto supplemented agar types, in four different tests, at room temperature.

FINDINGS

At the end of all tests (4-6 months) all noncontaminated subcultures were viable and maintained all phenotypic characteristics. Survival-time curves revealed a slow decrease of viable CFU over time on agar types, but at the end the number of viable CFU was satisfactory (≥2+ of growth). Decreasing of CFU was significantly higher for clinical versus nasopharyngeal isolates. Subcultures contamination rates were 6.25% and 14.58% after 2 and 6 months of storage, respectively.

CONCLUSION

TH-HYC and TH-SYC agar types allowed the viability of pneumococci with several serotypes, MLST, and genetic profiles, after 6 months of storage at room temperature. We consider that these agar types are a valid alternative to preserve pneumococci over an extended period, especially when methods as cryopreservation or lyophilization are not available, and are useful for transporting strains between laboratories.

摘要

目的

制备两种补充琼脂类型，以便在室温下储存肺炎球菌数月。

方法

使用 Todd-Hewitt/血红蛋白/酵母/活性炭/琼脂（TH-HYC）和 Todd-Hewitt/脱脂奶/酵母/活性炭/琼脂（TH-SYC）来制备两种补充琼脂类型。从显示不同血清型和多位点序列型（MLST）的患者或无症状携带者中分离出的 19 株肺炎球菌，经过传代培养并储存在补充琼脂类型上，在四个不同的测试中，在室温下进行。

结果

在所有测试结束时（4-6 个月），所有未污染的传代培养物均存活并保持所有表型特征。存活时间曲线显示，琼脂类型上的活菌 CFU 随时间缓慢减少，但最终存活的 CFU 数量令人满意（≥2+生长）。与鼻咽分离株相比，临床分离株的 CFU 减少率明显更高。储存 2 个月和 6 个月后，传代培养物的污染率分别为 6.25%和 14.58%。

结论

TH-HYC 和 TH-SYC 琼脂类型可在室温下储存 6 个月后保持多种血清型、MLST 和遗传特征的肺炎球菌的活力。我们认为，这些琼脂类型是一种有效的替代方法，可以在延长的时间内保存肺炎球菌，特别是在无法使用冷冻保存或冻干方法时，并且对于在实验室之间运输菌株非常有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/f05e176aed5d/BMRI2017-1218798.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/f1d4c753fcd4/BMRI2017-1218798.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/9d84454575ce/BMRI2017-1218798.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/47c37e4316da/BMRI2017-1218798.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/2b8530ccc92d/BMRI2017-1218798.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/38e2caaf0640/BMRI2017-1218798.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/f05e176aed5d/BMRI2017-1218798.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/f1d4c753fcd4/BMRI2017-1218798.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/9d84454575ce/BMRI2017-1218798.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/47c37e4316da/BMRI2017-1218798.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/2b8530ccc92d/BMRI2017-1218798.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/38e2caaf0640/BMRI2017-1218798.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c12/5735590/f05e176aed5d/BMRI2017-1218798.006.jpg

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