Talkington D F, Thacker W L, Keller D W, Jensen J S
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1998 Apr;36(4):1151-3. doi: 10.1128/JCM.36.4.1151-1153.1998.
A nested PCR specific for the Mycoplasma pneumoniae P1 gene was used to diagnose mycoplasma infection in two cohort patients with severe pneumonia within 24 h of tissue receipt. A postmortem diagnosis of M. pneumoniae infection was obtained for the first patient, who died without the collection of appropriate paired samples for serodiagnosis. An open-lung biopsy obtained from the second patient allowed a quick, definitive diagnosis and proper selection of therapy.
采用针对肺炎支原体P1基因的巢式聚合酶链反应（nested PCR），在收到组织样本后的24小时内对两组重症肺炎患者进行支原体感染诊断。首例患者在未采集用于血清学诊断的合适配对样本的情况下死亡，尸检诊断为肺炎支原体感染。从第二例患者获取的开胸肺活检样本实现了快速、明确的诊断并指导了恰当的治疗选择。
