通过巢式聚合酶链反应对尸检和开胸肺活检组织中的肺炎支原体感染进行诊断。
Diagnosis of Mycoplasma pneumoniae infection in autopsy and open-lung biopsy tissues by nested PCR.
作者信息
Talkington D F, Thacker W L, Keller D W, Jensen J S
机构信息
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1998 Apr;36(4):1151-3. doi: 10.1128/JCM.36.4.1151-1153.1998.
Abstract
A nested PCR specific for the Mycoplasma pneumoniae P1 gene was used to diagnose mycoplasma infection in two cohort patients with severe pneumonia within 24 h of tissue receipt. A postmortem diagnosis of M. pneumoniae infection was obtained for the first patient, who died without the collection of appropriate paired samples for serodiagnosis. An open-lung biopsy obtained from the second patient allowed a quick, definitive diagnosis and proper selection of therapy.
摘要
采用针对肺炎支原体P1基因的巢式聚合酶链反应（nested PCR），在收到组织样本后的24小时内对两组重症肺炎患者进行支原体感染诊断。首例患者在未采集用于血清学诊断的合适配对样本的情况下死亡，尸检诊断为肺炎支原体感染。从第二例患者获取的开胸肺活检样本实现了快速、明确的诊断并指导了恰当的治疗选择。
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