健康受试者和哮喘患者在痰液诱导过程中痰液成分的变化。

Changes in sputum composition during sputum induction in healthy and asthmatic subjects.

作者信息

Holz O, Jörres R A, Koschyk S, Speckin P, Welker L, Magnussen H

机构信息

Krankenhaus Grosshansdorf, Zentrum für Pneumologie und Thoraxchirurgie, LVA Freie und Hansestadt Hamburg, Germany.

出版信息

Clin Exp Allergy. 1998 Mar;28(3):284-92. doi: 10.1046/j.1365-2222.1998.00243.x.

DOI:10.1046/j.1365-2222.1998.00243.x

PMID:9543077

Abstract

BACKGROUND

Induced sputum is increasingly used to characterize the cellular and biochemical composition of the airways.

OBJECTIVE

We studied whether the composition of induced sputum is different between samples obtained sequentially during one sputum induction.

METHODS

Subjects with mild asthma (n=7) or healthy subjects (n=6) produced sputum during and after three consecutive 10 min periods of hypertonic saline inhalation. Samples were analysed separately for the three periods. To determine the reproducibility of the cellular composition, sputum induction was repeated on another two days.

RESULTS

The mean percentage of neutrophils decreased significantly (P<0.01) during sputum induction in asthmatic (36.9, 29.8, 16.3%) and healthy subjects (43.6, 17.2, 18.0%). Correspondingly, percentages of macrophages increased and percentages of eosinophils were 4.9, 3.5, and 3.7% in the asthmatic and 0.6, 0.7, and 0.5% in the healthy subjects, without significant change over the three periods; mean eosinophil numbers were significantly higher in the subjects with asthma (P< 0.05). Reproducibility of percentage cell counts did not markedly depend on sampling periods in terms of coefficients of variation. The concentration of eosinophil cationic protein decreased in both groups during sputum induction (P<0.01), geometric mean values being 579, 143, 57.4 microg L(-1) in the asthmatic and 130, 47.3, 28.4 microg L(-1) in the healthy subjects. Similar changes were seen for lactate dehydrogenase.

CONCLUSION

The separate analysis of induced sputum from three consecutive sampling periods of a single induction procedure demonstrated significant changes in their cellular and biochemical composition, both in healthy and mild asthmatic subjects.

摘要

背景

诱导痰越来越多地用于描述气道的细胞和生化组成。

目的

我们研究了在一次痰液诱导过程中连续获取的样本之间，诱导痰的组成是否存在差异。

方法

轻度哮喘患者（n = 7）或健康受试者（n = 6）在连续三个10分钟的高渗盐水吸入期间及之后咳出痰液。对这三个时间段的样本分别进行分析。为了确定细胞组成的可重复性，在另外两天重复进行痰液诱导。

结果

在哮喘患者（36.9%、29.8%、16.3%）和健康受试者（43.6%、17.2%、18.0%）的痰液诱导过程中，中性粒细胞的平均百分比显著下降（P<0.01）。相应地，巨噬细胞的百分比增加，哮喘患者的嗜酸性粒细胞百分比分别为4.9%、3.5%和3.7%，健康受试者为0.6%、0.7%和0.5%，在这三个时间段内无显著变化；哮喘患者的平均嗜酸性粒细胞数量显著更高（P<0.05）。就变异系数而言，细胞计数百分比的可重复性并不明显取决于采样时间段。两组在痰液诱导过程中嗜酸性粒细胞阳离子蛋白的浓度均下降（P<0.01），哮喘患者的几何平均值为579、143、57.4 μg L⁻¹，健康受试者为130、47.3、28.4 μg L⁻¹。乳酸脱氢酶也有类似变化。