Skopouli F N, Li L, Boumba D, Stefanaki S, Hanel K, Moutsopoulos H M, Krilis S A
Department of Internal Medicine, Medical School, University of Ioannina, Greece.
Clin Exp Rheumatol. 1998 Jan-Feb;16(1):63-5.
To determine the distribution and density of mast cells in the minor salivary glands of patients with primary Sjögren's syndrome (pSS) and of normal controls.
Minor salivary gland biopsies were obtained from 19 patients with pSS, 9 with systemic lupus erythematosus, one each with rheumatoid arthritis, sarcoidosis, and Hodgkin's disease, and from 10 individuals who had subjective xerostomia with normal salivary gland biopsies. Biopsy specimens were evaluated for the degree of inflammation according to Tarpley's classification. Sections were analysed for staining with Toluidine blue and with the mast cell specific marker c-kit. The data obtained were correlated with the histological findings of fatty infiltration, fibrosis and lymphocytic infiltration.
There was a significant correlation between the number of mast cells identified and the degrees of fibrosis and fatty infiltrates. There was no correlation between the intensity of lymphoid infiltration and the number of mast cells. c-kit staining showed a high correlation when compared to Toluidine blue staining.
Mast cells in the minor salivary glands of patients with pSS are strongly associated with fibrosis and cell acid infiltration. However, there is no correlation with parameters of disease activity such as lymphoid infiltration.
确定原发性干燥综合征（pSS）患者及正常对照者小唾液腺中肥大细胞的分布及密度。
获取19例pSS患者、9例系统性红斑狼疮患者、1例类风湿关节炎患者、1例结节病患者、1例霍奇金病患者以及10例有主观口干且唾液腺活检正常者的小唾液腺活检组织。根据塔尔普利分类法评估活检标本的炎症程度。对切片进行甲苯胺蓝染色及肥大细胞特异性标志物c-kit染色分析。将所得数据与脂肪浸润、纤维化及淋巴细胞浸润的组织学结果进行关联分析。
所识别的肥大细胞数量与纤维化及脂肪浸润程度之间存在显著相关性。淋巴细胞浸润强度与肥大细胞数量之间无相关性。与甲苯胺蓝染色相比，c-kit染色显示出高度相关性。
pSS患者小唾液腺中的肥大细胞与纤维化及细胞酸性浸润密切相关。然而，与诸如淋巴细胞浸润等疾病活动参数无相关性。
