Choudhury P, Kumar R
Division of Microbial Genetics, National Institute of Cholera and Enteric Diseases, India.
Can J Microbiol. 1998 Feb;44(2):186-9.
Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resistant to erythromycin (30 micrograms/mL), ampicillin (100 micrograms/mL), furazolidone (100 micrograms/mL), and penicillin (100 IU). These strains were able to grow in the presence of silver (Ag+), cobalt (Co2+), cadmium (Cd2+), nickel (Ni2+), lead (Pb2+), copper (Cu2+), and zinc (Zn2+) at concentrations up to 10 mM. All the strains showed similar plasmid profiles, ranging in sizes from 1.8 to 120 kb. Resistance to lead, cobalt, nickel, and copper was encoded by a 3.5-kb plasmid of K. pneumoniae. Synthesis of a 14-kDa periplasmic protein was increased when they were grown in presence of 10 mM Cu2+.
1996年季风来临前，从印度西孟加拉邦三角洲孙德尔本斯沿海地区（哈罗阿）采集了斑节对虾这种海洋虾类。从虾的消化道和鳃中分离出肺炎克雷伯菌，且为唯一分离菌株。所有10株分离菌株对红霉素（30微克/毫升）、氨苄青霉素（100微克/毫升）、呋喃唑酮（100微克/毫升）和青霉素（100国际单位）均有抗性。这些菌株能够在浓度高达10毫摩尔的银（Ag+）、钴（Co2+）、镉（Cd2+）、镍（Ni2+）、铅（Pb2+）、铜（Cu2+）和锌（Zn2+）存在的情况下生长。所有菌株都显示出相似的质粒图谱，大小在1.8至120千碱基之间。肺炎克雷伯菌的一个3.5千碱基的质粒编码了对铅、钴、镍和铜的抗性。当它们在10毫摩尔Cu2+存在的情况下生长时，一种14千道尔顿周质蛋白的合成增加。
