Simson D A, Ziemann F, Strigl M, Merkel R
Lehrstuhl für Biophysik, Technische Universität München, Garching, Germany.
Biophys J. 1998 Apr;74(4):2080-8. doi: 10.1016/S0006-3495(98)77915-9.
Measurements of forces in the piconewton range are very important for the study of molecular adhesion and mechanics. Recently, a micropipet-based force transducer for this type of experiment was presented (E. Evans, K. Ritchie, and R. Merkel, 1995, Biophys. J., 68:2580-2587). In the present article we give a detailed mechanical analysis of this transducer, including nonlinear effects. An analytical expression for the transducer stiffness at small elongations is given. Using magnetic tweezers (F. Ziemann, J. Rädler, and E. Sackmann, 1994, Biophys. J., 66:2210-2216), we were able to determine the force displacement relation of this transducer experimentally. Forces from approximately 10 pN to 500 pN were applied. Theoretical predictions and experimental results coincide remarkably well.
皮牛顿范围内力的测量对于分子粘附和力学研究非常重要。最近，有人提出了一种用于此类实验的基于微吸管的力传感器（E. 埃文斯、K. 里奇和R. 默克尔，1995年，《生物物理杂志》，68:2580 - 2587）。在本文中，我们对该传感器进行了详细的力学分析，包括非线性效应。给出了小伸长量下传感器刚度的解析表达式。使用磁镊（F. 齐曼、J. 勒德勒和E. 萨克曼，1994年，《生物物理杂志》，66:2210 - 2216），我们能够通过实验确定该传感器的力 - 位移关系。施加的力范围约为10皮牛顿至500皮牛顿。理论预测与实验结果非常吻合。
