Šmít Daniel, Fouquet Coralie, Doulazmi Mohamed, Pincet Frédéric, Trembleau Alain, Zapotocky Martin
Institute of Physiology, Czech Academy of Sciences, Vídeňská 1083, Prague, 14220 Czech Republic.
Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Kateřinská 2, Prague, 12000 Czech Republic.
BMC Biophys. 2017 Feb 13;10:2. doi: 10.1186/s13628-016-0033-2. eCollection 2017.
The Biomembrane Force Probe is an approachable experimental technique commonly used for single-molecule force spectroscopy and experiments on biological interfaces. The technique operates in the range of forces from 0.1 pN to 1000 pN. Experiments are typically repeated many times, conditions are often not optimal, the captured video can be unstable and lose focus; this makes efficient analysis challenging, while out-of-the-box non-proprietary solutions are not freely available.
This dedicated tool was developed to integrate and simplify the image processing and analysis of videomicroscopy recordings from BFP experiments. A novel processing feature, allowing the tracking of the pipette, was incorporated to address a limitation of preceding methods. Emphasis was placed on versatility and comprehensible user interface implemented in a graphical form.
An integrated analytical tool was implemented to provide a faster, simpler and more convenient way to process and analyse BFP experiments.
生物膜力探针是一种易于使用的实验技术，常用于单分子力谱和生物界面实验。该技术在0.1皮牛至1000皮牛的力范围内运行。实验通常会重复多次，条件往往不理想，捕获的视频可能不稳定且失焦；这使得高效分析具有挑战性，同时现成的非专有解决方案并非免费可得。
开发了这个专用工具，以整合和简化生物膜力探针实验视频显微镜记录的图像处理和分析。纳入了一项新颖的处理功能，允许跟踪移液器，以解决先前方法的局限性。重点在于以图形形式实现的通用性和易于理解的用户界面。
实施了一个集成分析工具，以提供一种更快、更简单、更方便的方式来处理和分析生物膜力探针实验。
