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固定红细胞的低频电旋转

Low frequency electrorotation of fixed red blood cells.

作者信息

Georgieva R, Neu B, Shilov V M, Knippel E, Budde A, Latza R, Donath E, Kiesewetter H, Bäumler H

机构信息

Institute of Transfusion Medicine and Immunohematology, Charité, Humboldt-University of Berlin, Germany.

出版信息

Biophys J. 1998 Apr;74(4):2114-20. doi: 10.1016/S0006-3495(98)77918-4.

Abstract

Electrorotation of fixed red blood cells has been investigated in the frequency range between 16 Hz and 30 MHz. The rotation was studied as a function of electrolyte conductivity and surface charge density. Between 16 Hz and 1 kHz, fixed red blood cells undergo cofield rotation. The maximum of cofield rotation occurs between 30 and 70 Hz. The position of the maximum depends weakly on the bulk electrolyte conductivity and surface charge density. Below 3.5 mS/m, the cofield rotation peak is broadened and shifted to higher frequencies accompanied by a decrease of the rotation speed. Surface charge reduction leads to a decrease of the rotation speed in the low frequency range. These observations are consistent with the recently developed electroosmotic theory of low frequency electrorotation.

摘要

已在16赫兹至30兆赫的频率范围内研究了固定红细胞的介电电泳。研究了旋转与电解质电导率和表面电荷密度的函数关系。在16赫兹至1千赫之间,固定红细胞发生同场旋转。同场旋转的最大值出现在30至70赫兹之间。最大值的位置对本体电解质电导率和表面电荷密度的依赖性较弱。低于3.5毫西门子/米时,同场旋转峰变宽并向更高频率移动,同时旋转速度降低。表面电荷减少导致低频范围内旋转速度降低。这些观察结果与最近发展的低频介电电泳电渗理论一致。

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