Techel D, Häfker T, Muschner S, Reimann M, Li Y, Monnerjahn C, Rensing L
Institute of Cell Biology, Biochemistry and Biotechnology, University of Bremen, Germany.
Biochim Biophys Acta. 1998 Apr 1;1397(1):21-6. doi: 10.1016/s0167-4781(98)00005-0.
The nucleotide sequence of the gene encoding the glucose-regulated protein 78 (GRP78) of Neurospora crassa was determined. The ORF codes for a protein of 662 amino acids (72 kDa) and belongs to the heat shock protein 70 (hsp70) gene family, which is characterized by three HSP70 'signature sequences'. The grp78 gene contains 5 introns. The protein carries the ER retention signal HDEL at its carboxy terminus and is most homologous to the KAR2/GRP78 protein of Saccharomyces cerevisiae (78%) and to KAR2/BiP of Yarrowia lipolytica (76%). The expression of grp78 is constitutive and can be enhanced by starvation, treatment with tunicamycin, the calcium ionophore A23187 or elevated temperatures (40 degrees C). An uninterrupted ORF was found on the reverse cDNA strand of grp78. The putative peptide shows 47% homology to the NAD-specific glutamate dehydrogenase of Achlya klebsiana.
测定了粗糙脉孢菌编码葡萄糖调节蛋白78（GRP78）的基因的核苷酸序列。该开放阅读框编码一个662个氨基酸（72 kDa）的蛋白质，属于热休克蛋白70（hsp70）基因家族，其特征是具有三个HSP70“特征序列”。grp78基因包含5个内含子。该蛋白质在其羧基末端带有内质网滞留信号HDEL，与酿酒酵母的KAR2/GRP78蛋白（78%）和解脂耶氏酵母的KAR2/BiP蛋白（76%）最为同源。grp78的表达是组成型的，饥饿、用衣霉素处理、钙离子载体A23187处理或温度升高（40摄氏度）均可增强其表达。在grp78的反向cDNA链上发现了一个不间断的开放阅读框。推测的肽与克氏壶菌的NAD特异性谷氨酸脱氢酶有47%的同源性。
