Kim-Shapiro D B, King S B, Bonifant C L, Kolibash C P, Ballas S K
Department of Physics, Wake Forest University, Winston-Salem, NC 27109-7507, USA.
Biochim Biophys Acta. 1998 Mar 12;1380(1):64-74. doi: 10.1016/s0304-4165(97)00132-3.
Hydroxyurea has been mixed with hemoglobin S and the reaction was studied using electronic absorption spectroscopy as a function of time and wavelength. The rate of conversion of oxyhemoglobin S to other species was determined and the nature of the reaction products was studied. We also report the formation of methemoglobin (and other reaction products) when deoxyhemoglobin S is combined with hydroxyurea. The probable increase in the formation of methemoglobin, and other potential reaction products such as nitric oxide-hemoglobin, in patients with sickle cell anemia who are taking hydroxyurea as a therapeutic drug is discussed in terms of the pathophysiology of the disease. It is proposed that methemoglobin and possibly nitric oxide-hemoglobin formation may partially explain beneficial effects observed in these patients before their levels of fetal hemoglobin have increased.
已将羟基脲与血红蛋白S混合，并使用电子吸收光谱法研究了该反应随时间和波长的变化情况。测定了氧合血红蛋白S向其他物种的转化速率，并研究了反应产物的性质。我们还报告了脱氧血红蛋白S与羟基脲结合时高铁血红蛋白（及其他反应产物）的形成情况。从该疾病的病理生理学角度讨论了镰状细胞贫血患者服用羟基脲作为治疗药物时高铁血红蛋白及其他潜在反应产物（如一氧化氮 - 血红蛋白）形成可能增加的情况。有人提出，高铁血红蛋白以及可能的一氧化氮 - 血红蛋白的形成可能部分解释了在这些患者胎儿血红蛋白水平升高之前观察到的有益效果。
