Tay F R, Gwinnett A J, Wei S H
Department of Children's Dentistry and Orthodontics, Prince Philip Dental Hospital, University of Hong Kong.
Am J Dent. 1997 Apr;10(2):77-82.
This study investigated the effect of reversible and irreversible rewetting on the application of One-Step, single-bottle, water-free, acetone-based primer/adhesive.
Thirty 1 mm dentin discs prepared from third molars were each conditioned with 10% H3PO4 for 20 seconds and rinsed for 20 seconds. They were randomly divided into five groups: Group 1 (positive control): conditioned dentin bonded visibly moist; Group 2: bonded after drying for 3 seconds; Group 3: drying for 3 seconds and rewetting with distilled water; Group 4 (negative control): drying for 3 seconds, stored in 8% glutaraldehyde for 15 minutes, rinsed with distilled water and dried for 3 seconds; and Group 5: drying for 3 seconds, stored in glutaraldehyde for 15 minutes, rinsed with distilled water and bonded visibly moist. One-Step was applied in two coats according to the manufacturer's recommendation and discs in each group were further bonded together to form a disc-pair using a chemical cure resin, demineralized in EDTA and prepared for TEM examination.
Complete resin infiltration into the zone of demineralized dentin was observed in Group 1 and 3. In Group 2, resin infiltration was limited to the surface and base of the demineralized network apparently along the lateral branches of the dentin tubules. A middle zone of suboptimal infiltration was observed. In Group 4 and 5, minimal resin infiltration was only observed along the base of the demineralized network upon their fixation in a collapsed state with glutaraldehyde.
本研究调查了可逆和不可逆再湿对一步法、单瓶、无水、丙酮基底漆/粘合剂应用的影响。
从第三磨牙制备的30个1毫米牙本质圆盘，每个用10%磷酸处理20秒，冲洗20秒。它们被随机分为五组：第1组（阳性对照）：处理后的牙本质在明显湿润时粘结；第2组：干燥3秒后粘结；第3组：干燥3秒后用蒸馏水再湿；第4组（阴性对照）：干燥3秒，在8%戊二醛中储存15分钟，用蒸馏水冲洗并干燥3秒；第5组：干燥3秒，在戊二醛中储存15分钟，用蒸馏水冲洗并在明显湿润时粘结。按照制造商的建议分两层涂抹一步法，每组中的圆盘进一步用化学固化树脂粘结在一起形成圆盘对，在乙二胺四乙酸中脱矿并准备进行透射电子显微镜检查。
在第1组和第3组中观察到树脂完全渗入脱矿牙本质区域。在第2组中，树脂渗入仅限于脱矿网络的表面和底部，明显沿着牙本质小管的侧支。观察到一个渗透不理想的中间区域。在第4组和第5组中，在用戊二醛固定在塌陷状态后，仅在脱矿网络底部观察到最小限度的树脂渗入。
