Department of Endodontics, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.
Department of Endodontics, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.
J Endod. 2017 Dec;43(12):2088-2092. doi: 10.1016/j.joen.2017.07.016. Epub 2017 Oct 9.
Many endodontic sealers are available, but the search for the ideal sealer continues. This study evaluated the cytotoxicity and biocompatibility of Sealer Plus, a new resin epoxy-based endodontic sealer containing calcium hydroxide. AH Plus, Endofill, and SimpliSeal endodontics sealers were used for comparison.
L929 fibroblasts were cultured, and an MTT assay was used to determine the cytotoxicity of the sealer extracts at 6, 24, 48, and 72 hours. Tubes containing materials or empty tubes for control were inserted into the subcutaneous tissues of 20 rats. After 7 and 30 days, the rats were killed, and the tubes were removed with the surrounding tissues for histologic analysis. The data were submitted to statistical tests (P < .05).
Undiluted Sealer Plus exhibited less cytotoxicity compared with other undiluted extracts at 6 hours (P < .05), and cell viability was higher for all Sealer Plus extracts after 24 hours (P < .05). At 48 hours, the undiluted and ½ Sealer Plus dilution were the extracts with less cytotoxicity (P < .05). At 72 hours, cell viability was higher for the undiluted and ½ Sealer Plus dilution compared with the other sealers (P < .05). At 7 days, Endofill and SimpliSeal had higher inflammation compared with the control and Sealer Plus (P < .05); AH Plus had moderate inflammation (P > .05). At 30 days, control, Sealer Plus, and AH Plus had less inflammation (P < .05). The fibrous capsule was thick at 7 days and thin at 30 days, except for SimpliSeal.
In general, Sealer Plus promoted greater cell viability and was more biocompatible compared with the other sealers.
有许多种根管封闭剂可供选择，但人们仍在不断寻找理想的封闭剂。本研究评估了一种新型树脂环氧基根管封闭剂 Sealer Plus 的细胞毒性和生物相容性，该封闭剂含有氢氧化钙。AH Plus、Endofill 和 SimpliSeal 根管封闭剂被用于比较。
培养 L929 成纤维细胞，使用 MTT 法在 6、24、48 和 72 小时测定封闭剂浸提液的细胞毒性。将装有材料的管或空白管（对照）插入 20 只大鼠的皮下组织中。7 和 30 天后，处死大鼠，取出带有周围组织的管进行组织学分析。将数据提交给统计检验（P<.05）。
未稀释的 Sealer Plus 在 6 小时时与其他未稀释提取物相比细胞毒性更小（P<.05），并且所有 Sealer Plus 提取物在 24 小时后细胞活力更高（P<.05）。在 48 小时时，未稀释和 1/2 Sealer Plus 稀释是细胞毒性最小的提取物（P<.05）。在 72 小时时，未稀释和 1/2 Sealer Plus 稀释的细胞活力高于其他封闭剂（P<.05）。在 7 天时，Endofill 和 SimpliSeal 与对照组和 Sealer Plus 相比炎症反应更高（P<.05）；AH Plus 有中度炎症（P>.05）。在 30 天时，对照组、Sealer Plus 和 AH Plus 的炎症反应较低（P<.05）。纤维囊在 7 天时较厚，在 30 天时较薄，但 SimpliSeal 除外。
总的来说，与其他封闭剂相比，Sealer Plus 促进了更高的细胞活力和更好的生物相容性。
