Liang R, Chan W P, Kwong Y L, Chan A C, Xu W S, Srivastava G
Department of Medicine, University of Hong Kong, Queen Mary Hospital, People's Republic of China.
Cancer Genet Cytogenet. 1998 Apr 15;102(2):110-3. doi: 10.1016/s0165-4608(97)00353-1.
BCL-6 gene rearrangement and hypermutations were investigated in four Hong Kong Chinese patients with low-grade gastric lymphoma of the mucosa-associated lymphoid tissue (MALToma). The former was studied by Southern analysis and the latter by the technique of polymerase chain reaction and denaturing gradient gel electrophoresis. BCL-6 gene rearrangement was not detectable in any of the four cases. However, mutations at both the E1.11 and E1.12 segments of the 5' noncoding region of the BCL-6 gene were found in two patients. This preliminary observation suggests that the mutations of the 5' noncoding region of the BCL-6 gene rather than gene rearrangement may be playing a more important role in the tumorigenesis of low-grade gastric MALToma. Further confirmation of this finding by studying a larger number of patients will be required.
对4例香港中国黏膜相关淋巴组织低度胃淋巴瘤（MALToma）患者的BCL-6基因重排和高突变情况进行了研究。前者通过Southern分析进行研究，后者通过聚合酶链反应和变性梯度凝胶电泳技术进行研究。在这4例病例中均未检测到BCL-6基因重排。然而，在2例患者中发现了BCL-6基因5'非编码区E1.11和E1.12区段的突变。这一初步观察结果表明，BCL-6基因5'非编码区的突变而非基因重排在低度胃MALToma的肿瘤发生过程中可能发挥更重要的作用。需要通过研究更多患者来进一步证实这一发现。
