Thomas R A, Lawlor K, Bailey M, Macaskie L E
School of Biological Sciences, University of Birmingham, United Kingdom.
Appl Environ Microbiol. 1998 Apr;64(4):1319-22. doi: 10.1128/AEM.64.4.1319-1322.1998.
A mixed culture utilizing EDTA as the sole carbon source was isolated from a mixed inoculum of water from the River Mersey (United Kingdom) and sludge from an industrial effluent treatment plant. Fourteen component organisms were isolated from the culture, including representatives of the genera Methylobacterium, Variovorax, Enterobacter, Aureobacterium, and Bacillus. The mixed culture biodegraded metal-EDTA complexes slowly; the biodegradability was in the order Fe > Cu > Co > Ni > Cd. By incorporation of inorganic phosphate into the medium as a precipitant ligand, heavy metals were removed in parallel to EDTA degradation. The mixed culture also utilized a number of possible EDTA degradation intermediates as carbon sources.
从英国默西河的水样和工业废水处理厂的污泥混合接种物中分离出一种以乙二胺四乙酸（EDTA）作为唯一碳源的混合培养物。从该培养物中分离出14种组成生物体，包括甲基杆菌属、贪铜菌属、肠杆菌属、金色杆菌属和芽孢杆菌属的代表菌株。该混合培养物对金属-EDTA络合物的生物降解缓慢；生物降解性顺序为铁＞铜＞钴＞镍＞镉。通过向培养基中添加无机磷酸盐作为沉淀配体，重金属随着EDTA的降解而被去除。该混合培养物还利用多种可能的EDTA降解中间产物作为碳源。
