Technical Chemistry and Chemical Engineering, University of Paderborn, Warburger Strasse 100, D-4790 Paderborn, Germany.
Appl Environ Microbiol. 1992 Feb;58(2):671-6. doi: 10.1128/aem.58.2.671-676.1992.
A bacterial mixed culture, which was obtained from sewage by a special enrichment procedure, utilized EDTA as the sole source of carbon and nitrogen for growth. High concentrations of mineral salts, particularly CaCl(2), or the use of a mineral base without nitrogen protected the cells from inactivation after transfer into fresh medium containing 200-mg/liter (0.67 mM) EDTA. The chemical speciation did not influence the biodegradability of EDTA. However, when resting cells of the mixed culture were incubated with EDTA in the presence of an equivalent molar amount of FeCl(3), the reaction came to a halt before the complete consumption of the substrate. A gram-negative isolate from the mixed population, BNC1, also metabolized EDTA in monoculture. Growth of the pure culture was promoted by biotin or folic acid but was always accompanied by the accumulation of unidentified metabolites and was slow (mu(max), 0.024 h) compared with that of the original community (mu(max), 0.036 h).
一种从污水中通过特殊富集程序获得的细菌混合培养物，将 EDTA 用作生长的唯一碳源和氮源。高浓度的矿物质盐，特别是 CaCl(2)，或使用不含氮的矿物质基础，可以防止细胞在转移到含有 200 毫克/升（0.67 毫摩尔）EDTA 的新鲜培养基中后失活。化学形态不会影响 EDTA 的生物降解性。然而，当混合培养物的休止细胞在存在等量 FeCl(3)的情况下与 EDTA 孵育时，反应在底物完全消耗之前停止。从混合种群中分离出的革兰氏阴性菌 BNC1 也可以在纯培养中代谢 EDTA。生物素或叶酸促进纯培养物的生长，但总是伴随着未鉴定代谢物的积累，并且与原始群落（mu(max)，0.036 h）相比，生长速度较慢（mu(max)，0.024 h）。
