Künkel A, Vorholt J A, Thauer R K, Hedderich R
Max-Planck-Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Marburg, Germany.
Eur J Biochem. 1998 Mar 15;252(3):467-76. doi: 10.1046/j.1432-1327.1998.2520467.x.
Methanogenic archaea are known to contain two types of [NiFe] hydrogenases designated F420-reducing hydrogenase and F420-non-reducing hydrogenase. We report here that they additionally contain Escherichia coli hydrogenase-3-type [NiFe] hydrogenases. The evidence is based on biochemical studies and analysis of the subunit primary structure of this hydrogenase (designated Ech) purified from membranes of acetate-grown cells of Methanosarcina barkeri. The subunits EchE and EchC of the EchABCDEF complex showed 34% and 45% sequence identity to the nickel-containing large subunit HycE and to the iron-sulfur cluster containing small subunit HycG, respectively, of the hydrogenase in the formate hydrogen lyase complex from E. coli. Analysis of the totally sequenced genomes of Methanococcus jannaschii and Methanobacterium thermoautotrophicum strain deltaH revealed that these organisms contain similar open reading frames, indicating the presence of an E. coli hydrogenase-3-type hydrogenase also in these methanogenic archaea.
已知产甲烷古菌含有两种类型的[NiFe]氢化酶，分别称为F420还原氢化酶和F420非还原氢化酶。我们在此报告，它们还含有大肠杆菌氢化酶-3型[NiFe]氢化酶。证据基于对从巴氏甲烷八叠球菌乙酸盐培养细胞的膜中纯化的这种氢化酶（称为Ech）的亚基一级结构的生化研究和分析。EchABCDEF复合物的亚基EchE和EchC与来自大肠杆菌的甲酸氢裂解酶复合物中的氢化酶的含镍大亚基HycE和含铁硫簇的小亚基HycG的序列同一性分别为34%和45%。对詹氏甲烷球菌和嗜热自养甲烷杆菌δH菌株的全序列基因组分析表明，这些生物体含有相似的开放阅读框，表明在这些产甲烷古菌中也存在大肠杆菌氢化酶-3型氢化酶。
