Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
Adv Biochem Eng Biotechnol. 2022;180:57-90. doi: 10.1007/10_2021_199.
With a presence of the Wood-Ljungdahl pathway, acetogenic bacteria are capable of converting C1 feedstocks into biomass and various metabolites, receiving industrial interest in microbial production of biochemicals derived from C1 substrates. To understand C1 feedstock fermentation using acetogenic bacteria, most of the studies have focused on revealing their carbon assimilation and energy conservation systems. Despite the determination of the essential mechanisms, a fundamental understanding of acetogenic bacteria and the associated complex regulatory systems remains unclear and is needed for rational strain design. For this purpose, systems biology is a suitable approach for investigating genome, transcription, translation, regulation systems, and metabolic flux, providing a glimpse of the relationship between the genotype and phenotype of the organisms. This chapter will cover recent systems biology applications on acetogenic bacteria and discuss the cellular responses during C1 feedstock fermentation along with the regulatory systems that orchestrate cellular processes.
具有 Wood-Ljungdahl 途径的产乙酸菌能够将 C1 原料转化为生物质和各种代谢物，这使其在微生物生产源自 C1 底物的生物化学物质方面受到了工业关注。为了了解产乙酸菌利用 C1 原料进行发酵，大多数研究都集中在揭示其碳同化和能量守恒系统上。尽管已经确定了基本机制，但对产乙酸菌及其相关复杂调控系统的基本了解仍不清楚，这对于合理的菌株设计是必要的。为此，系统生物学是研究基因组、转录、翻译、调控系统和代谢通量的合适方法，为了解生物体的基因型和表型之间的关系提供了一个视角。本章将介绍产乙酸菌的系统生物学应用，并讨论在 C1 原料发酵过程中的细胞响应以及协调细胞过程的调控系统。
