Schneider J, Gilbert S C, Blanchard T J, Hanke T, Robson K J, Hannan C M, Becker M, Sinden R, Smith G L, Hill A V
Institute of Molecular Medicine, Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, UK.
Nat Med. 1998 Apr;4(4):397-402. doi: 10.1038/nm0498-397.
Immunization with irradiated sporozoites can protect against malaria infection and intensive efforts are aimed at reproducing this effect with subunit vaccines. A particular sequence of subunit immunization with pre-erythrocytic antigens of Plasmodium berghei, consisting of single dose priming with plasmid DNA followed by a single boost with a recombinant modified vaccinia virus Ankara (MVA) expressing the same antigen, induced unprecedented complete protection against P. berghei sporozoite challenge in two strains of mice. Protection was associated with very high levels of splenic peptide-specific interferon-gamma-secreting CD8+ T cells and was abrogated when the order of immunization was reversed. DNA priming followed by MVA boosting may provide a general immunization regime for induction of high levels of CD8+ T cells.
用辐照子孢子进行免疫可预防疟疾感染，目前正致力于用亚单位疫苗重现这种效果。对伯氏疟原虫的红细胞前期抗原进行亚单位免疫的特定序列，即先用质粒DNA单剂量启动，然后用表达相同抗原的重组改良安卡拉痘苗病毒（MVA）进行单次加强免疫，在两株小鼠中诱导出了前所未有的针对伯氏疟原虫子孢子攻击的完全保护作用。这种保护作用与脾脏中高水平的肽特异性分泌干扰素-γ的CD8+T细胞有关，当免疫顺序颠倒时，这种保护作用就会消失。先用DNA启动，然后用MVA加强免疫，可能为诱导高水平的CD8+T细胞提供一种通用的免疫方案。
