Campbell J M, Payne A P, Gilmore D P, Russell D, McGadey J, Clarke D J, Branton R, Davies R W, Sutcliffe R G
Division of Neuroscience, Institute of Biomedical and Life Sciences, Glasgow University, UK.
Neurosci Lett. 1997 Dec 12;239(1):54-6. doi: 10.1016/s0304-3940(97)00871-9.
The AS/AGU rat is characterised by an ungainly, staggering gait, hind-limb rigidity, whole body tremor and (in older animals) difficulty in initiating movement. Brains of AS and AS/AGU males aged between 3 and 12 months (n = 10 per group) were sectioned transversely on a cryostat (-20 degrees C) to produce two successive cut faces (corresponding approximately to Bregma +1.2 and -0.5 mm) and 1 mm diameter x 1 mm deep micropunches were taken from four areas of the caudate-putamen. Levels of dopamine in all four areas (measured by HPLC-ECD followed by protein estimation) peaked at around 6 months and then declined in AS and AS/AGU rats. In the dorsal and lateral caudate-putamen, dopamine levels were significantly reduced in AS/AGU rats compared to AS controls from 6 months onwards. This provides further evidence that the AS/AGU mutant has impairment of its striatal dopaminergic systems.
AS/AGU大鼠的特征是步态笨拙、蹒跚，后肢僵硬，全身震颤，并且（在老年动物中）起始运动困难。对3至12个月大的AS和AS/AGU雄性大鼠（每组n = 10）的大脑在低温恒温器（-20摄氏度）上进行横向切片，以产生两个连续的切面（大致对应于前囟+1.2和-0.5毫米），并从尾状核-壳核的四个区域取出直径1毫米×深1毫米的微穿孔样本。通过高效液相色谱-电化学检测法（HPLC-ECD）并随后进行蛋白质定量，测量所有四个区域的多巴胺水平，结果显示在AS和AS/AGU大鼠中，多巴胺水平在大约6个月时达到峰值，然后下降。在背侧和外侧尾状核-壳核中，从6个月起，与AS对照组相比，AS/AGU大鼠的多巴胺水平显著降低。这进一步证明AS/AGU突变体的纹状体多巴胺能系统存在损伤。
