分子信标探针与核酸序列扩增技术（NASBA）相结合，能够实现对RNA的均相实时检测。
Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA.
作者信息
Leone G, van Schijndel H, van Gemen B, Kramer F R, Schoen C D
机构信息
DLO Research Institute for Plant Protection (IPO-DLO), PO Box 9060, 6700 GW Wageningen, The Netherlands.
出版信息
Nucleic Acids Res. 1998 May 1;26(9):2150-5. doi: 10.1093/nar/26.9.2150.
Abstract
Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize within the target sequence, was introduced into NASBA reactions that amplify the genomic RNA of potato leafroll virus (PLRV). During amplification, the probe anneals to the antisense RNA amplicon generated by NASBA, producing a specific fluorescent signal that can be monitored in real-time. The assay is rapid, sensitive and specific. As RNA amplification and detection can be carried out in unopened vessels, it minimizes the risk of carry-over contaminations. Robustness has been verified on real-world samples. This homogeneous assay, called AmpliDet RNA, is a significant improvement over current detection methods for NASBA amplicons and is suitable for one-tube applications ranging from high-throughput diagnostics to in vivo studies of biological activities.
摘要
分子信标探针可用于核酸序列扩增技术（NASBA）扩增子检测系统，以便在扩增过程中同时产生特定的荧光信号。将一种设计用于与靶序列杂交的分子信标引入到NASBA反应中，该反应可扩增马铃薯卷叶病毒（PLRV）的基因组RNA。在扩增过程中，探针与NASBA产生的反义RNA扩增子退火，产生可实时监测的特定荧光信号。该检测方法快速、灵敏且特异。由于RNA扩增和检测可在未开封的容器中进行，因此将交叉污染的风险降至最低。已在实际样本中验证了其稳健性。这种称为AmpliDet RNA的均相检测方法是对当前NASBA扩增子检测方法的重大改进，适用于从高通量诊断到生物活性体内研究的单管应用。
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Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA.分子信标探针与核酸序列扩增技术（NASBA）相结合，能够实现对RNA的均相实时检测。
Nucleic Acids Res. 1998 May 1;26(9):2150-5. doi: 10.1093/nar/26.9.2150.
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