Abou-Seif M A
Chemistry Department, Faculty of Science, Mansoura University, Egypt.
Ann Clin Biochem. 1998 Mar;35 ( Pt 2):254-60. doi: 10.1177/000456329803500209.
It has been suggested that aluminium stimulates vanadium-mediated superoxide radical generation. The oxidative stress of generated superoxide radicals on antioxidant enzyme activity, oxidation of NADH and NADPH, membrane lipid peroxidation and osmotic fragility in human red blood cells (RBC) was investigated. RBC were incubated with varying concentrations of vanadium and aluminium ions at 37 degrees C for 2 h. RBC incubated with vanadium ions showed significantly increased superoxide dismutase and catalase activities, and oxidized NADH and NADPH concentrations compared with control RBC preparations. Erythrocyte lipid peroxidation was assessed by measuring thiobarbituric acid reactivity. RBC incubated with elevated levels of vanadium showed significantly increased membrane lipid peroxidation when compared with control RBC; it increased further on addition of aluminium. A significant positive correlation was observed between the extent of vanadium induced membrane lipid peroxidation and the osmotic fragility of treated RBC. In the presence of vanadium, aluminium stimulates superoxide dismutase and catalase activities. NADH and NADPH oxidation and membrane lipid peroxidation, as well as increasing osmotic fragility of human erythrocytes. The stimulatory effect of aluminium was dependent on concentration. These results may have implications for the mechanism of toxicity of aluminium and vanadium in haemodialysis patients.
有人提出铝会刺激钒介导的超氧自由基生成。研究了所生成的超氧自由基对人红细胞（RBC）抗氧化酶活性、NADH和NADPH氧化、膜脂质过氧化及渗透脆性的氧化应激作用。将红细胞在37℃下与不同浓度的钒离子和铝离子孵育2小时。与对照红细胞制剂相比，用钒离子孵育的红细胞超氧化物歧化酶和过氧化氢酶活性显著增加，NADH和NADPH氧化浓度升高。通过测量硫代巴比妥酸反应性评估红细胞脂质过氧化。与对照红细胞相比，用高水平钒孵育的红细胞膜脂质过氧化显著增加；加入铝后进一步增加。观察到钒诱导的膜脂质过氧化程度与处理后红细胞的渗透脆性之间存在显著正相关。在钒存在的情况下，铝会刺激超氧化物歧化酶和过氧化氢酶活性。NADH和NADPH氧化以及膜脂质过氧化，同时增加人红细胞的渗透脆性。铝的刺激作用取决于浓度。这些结果可能对血液透析患者中铝和钒的毒性机制具有启示意义。
