Jain S K
Department of Pediatrics, Louisiana State University School of Medicine, Shreveport 71130.
J Biol Chem. 1989 Dec 15;264(35):21340-5.
The present study has examined the effect of elevated glucose levels on membrane lipid peroxidation and osmotic fragility in human red blood cells (RBC). Defibrinated whole blood or RBC were incubated with varying concentrations of glucose at 37 degrees C for 24 h. RBC incubated with elevated levels of glucose showed a significantly increased membrane lipid peroxidation when compared with control RBC. A significant positive correlation was observed between the extent of glucose-induced membrane lipid peroxidation and the osmotic fragility of treated RBC. Glucose-induced membrane lipid peroxidation and osmotic fragility were blocked when RBC were pretreated with fluoride, an inhibitor of glucose metabolism; with vitamin E, an antioxidant; with para-chloromercurobenzoate and metyrapone, inhibitors of the cytochrome P-450 system; or with dimethylfurane, diphenylamine, and thiourea, scavengers of oxygen radicals. RBC treated with elevated glucose concentrations also showed an increase in NADPH levels. Exogenous addition of NADPH to normal RBC lysate induced membrane lipid peroxidation similar to that observed in the glucose-treated RBC. These data suggest that elevated glucose levels can cause the peroxidation of membrane lipids in human RBC.
本研究检测了葡萄糖水平升高对人红细胞（RBC）膜脂质过氧化和渗透脆性的影响。将去纤维蛋白全血或红细胞与不同浓度的葡萄糖在37℃孵育24小时。与对照红细胞相比，用升高水平的葡萄糖孵育的红细胞显示出膜脂质过氧化显著增加。在葡萄糖诱导的膜脂质过氧化程度与处理后红细胞的渗透脆性之间观察到显著的正相关。当红细胞用氟化物（一种葡萄糖代谢抑制剂）、维生素E（一种抗氧化剂）、对氯汞苯甲酸和甲吡酮（细胞色素P-450系统抑制剂）或用二甲基呋喃、二苯胺和硫脲（氧自由基清除剂）预处理时，葡萄糖诱导的膜脂质过氧化和渗透脆性被阻断。用升高的葡萄糖浓度处理的红细胞还显示NADPH水平增加。向正常红细胞裂解物中额外添加NADPH会诱导膜脂质过氧化，类似于在葡萄糖处理的红细胞中观察到的情况。这些数据表明，葡萄糖水平升高可导致人红细胞膜脂质过氧化。
