高效液相色谱法测定人胆汁中的游离脂肪酸

Determination of free fatty acids in human bile by high-performance liquid chromatography.

作者信息

Hori Y, Nakamura K, Yamamoto M, Shimada K, Nakadaira H, Shibuya N, Endoh K, Ogoshi K

机构信息

Department of Hygiene and Preventive Medicine, Niigata University School of Medicine, Japan.

出版信息

Ann Clin Biochem. 1998 Mar;35 ( Pt 2):279-82. doi: 10.1177/000456329803500213.

DOI:10.1177/000456329803500213

PMID:9547901

Abstract

We developed a high-performance liquid chromatography (HPLC) method for free fatty acids (FFAs) analysis in bile. In this method, FFAs were extracted from bile in a single step using an Isolute ODS cartridge, derivatized with 9-anthryldiazomethane (ADAM). ADAM was chosen because of its high reactivity with carboxylic acid at room temperature. Then, HPLC was used for separating and quantifying FFAs. This method proved to be simple and time-saving. The mean recovery of FFA added to human gallbladder bile was 97.6%, and the detection limit was 100-250 pg. Using this method, we determined FFA concentrations in the gallbladder bile of 11 gallstone patients. The mean concentration of total FFA was 0.61 (SD = 0.41) mmol/L, and there was wide variation in the individual FFAs.

摘要

我们开发了一种用于分析胆汁中游离脂肪酸（FFA）的高效液相色谱（HPLC）方法。在该方法中，使用Isolute ODS柱从胆汁中一步提取FFA，并用9-蒽重氮甲烷（ADAM）进行衍生化。选择ADAM是因为它在室温下与羧酸具有高反应活性。然后，使用HPLC分离和定量FFA。该方法被证明是简单且省时的。添加到人体胆囊胆汁中的FFA的平均回收率为97.6%，检测限为100 - 250 pg。使用该方法，我们测定了11例胆结石患者胆囊胆汁中的FFA浓度。总FFA的平均浓度为0.61（标准差 = 0.41）mmol/L，并且各个FFA之间存在很大差异。