表达全长丙型肝炎病毒cDNA的转基因小鼠。
Transgenic mouse expressing a full-length hepatitis C virus cDNA.
作者信息
Matsuda J, Suzuki M, Nozaki C, Shinya N, Tashiro K, Mizuno K, Uchinuno Y, Yamamura K
机构信息
The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center, Pathology Department, Kumamoto.
出版信息
Jpn J Cancer Res. 1998 Feb;89(2):150-8. doi: 10.1111/j.1349-7006.1998.tb00543.x.
Abstract
Hepatitis C virus (HCV), a major causative agent of post transfusion non-A, non-B hepatitis (NANBH), can only infect humans and chimpanzees. We produced nine transgenic mouse lines carrying a full-length HCV cDNA with the human serum amyloid P component (hSAP) promoter that can direct liver-specific expression. In one of these lines HCV mRNA and HCV core protein were detected in the liver of the transgenic mouse, although the levels of expression were very low. In addition, HCV-related antibody was detected in the serum.
摘要
丙型肝炎病毒（HCV）是输血后非甲非乙型肝炎（NANBH）的主要致病因子，仅感染人类和黑猩猩。我们构建了九条携带全长HCV cDNA并由人血清淀粉样蛋白P成分（hSAP）启动子驱动的转基因小鼠品系，该启动子可指导肝脏特异性表达。在其中一个品系的转基因小鼠肝脏中检测到了HCV mRNA和HCV核心蛋白，尽管表达水平很低。此外，在血清中检测到了HCV相关抗体。
相似文献
1
Transgenic mouse expressing a full-length hepatitis C virus cDNA.表达全长丙型肝炎病毒cDNA的转基因小鼠。
Jpn J Cancer Res. 1998 Feb;89(2):150-8. doi: 10.1111/j.1349-7006.1998.tb00543.x.
2
Transgenic expression of hepatitis C virus structural proteins in the mouse.丙型肝炎病毒结构蛋白在小鼠中的转基因表达。
Hepatology. 1997 Apr;25(4):1014-21. doi: 10.1002/hep.510250437.
3
Expression of hepatitis C virus core protein associated with malignant lymphoma in transgenic mice.
Comp Immunol Microbiol Infect Dis. 2003 Mar;26(2):115-24. doi: 10.1016/s0147-9571(02)00038-3.
4
Transmission of HCV to a chimpanzee using virus particles produced in an RNA-transfected HepG2 cell culture.使用在RNA转染的HepG2细胞培养物中产生的病毒颗粒将丙型肝炎病毒传播给黑猩猩。
J Med Virol. 2001 Oct;65(2):276-81. doi: 10.1002/jmv.2030.
5
Serum hepatitis C virus sequences in posttransfusion non-A, non-B hepatitis.输血后非甲非乙型肝炎中的血清丙型肝炎病毒序列
Blood. 1991 Mar 15;77(6):1157-60.
6
Possible role of cytotoxic T cells in acute liver injury in hepatitis C virus cDNA transgenic mice mediated by Cre/loxP system.细胞毒性T细胞在由Cre/loxP系统介导的丙型肝炎病毒cDNA转基因小鼠急性肝损伤中的可能作用。
J Med Virol. 2000 Nov;62(3):308-17. doi: 10.1002/1096-9071(200011)62:3<308::aid-jmv2>3.0.co;2-6.
7
Diagnostic discordance for hepatitis C virus infection in hemodialysis patients.血液透析患者丙型肝炎病毒感染的诊断不一致性
Am J Kidney Dis. 2005 Aug;46(2):290-300. doi: 10.1053/j.ajkd.2005.05.006.
8
Circulating HCV-RNA, HCV genotype, and liver histology in asymptomatic individuals reactive for anti-HCV antibody and their follow-up study.抗丙型肝炎病毒（HCV）抗体反应性无症状个体的循环HCV-RNA、HCV基因型及肝脏组织学检查及其随访研究。
Liver. 1996 Aug;16(4):241-7. doi: 10.1111/j.1600-0676.1996.tb00736.x.
9
Improved detection of hepatitis C virus RNA by reverse transcription and polymerase chain reaction.通过逆转录和聚合酶链反应改进丙型肝炎病毒RNA的检测
Eur J Clin Chem Clin Biochem. 1992 Nov;30(11):717-27. doi: 10.1515/cclm.1992.30.11.717.
10
Hepatitis C virus infection in Jordanian haemodialysis units: serological diagnosis and genotyping.约旦血液透析单位中的丙型肝炎病毒感染：血清学诊断与基因分型
J Med Microbiol. 2002 Aug;51(8):700-704. doi: 10.1099/0022-1317-51-8-700.
引用本文的文献
1
Modeling Hepatotropic Viral Infections: Cells vs. Animals.肝炎病毒感染模型：细胞与动物。
Cells. 2021 Jul 8;10(7):1726. doi: 10.3390/cells10071726.
2
Reduced body weight and increased energy expenditure in transgenic mice over-expressing soluble leptin receptor.转可溶性瘦素受体基因小鼠体重减轻和能量消耗增加。
PLoS One. 2010 Jul 20;5(7):e11669. doi: 10.1371/journal.pone.0011669.
3
Generation of the regulatory protein rtTA transgenic mice.调控蛋白rtTA转基因小鼠的产生。
World J Gastroenterol. 2005 May 21;11(19):2885-91. doi: 10.3748/wjg.v11.i19.2885.
4
Repeated hepatocyte injury promotes hepatic tumorigenesis in hepatitis C virus transgenic mice.反复的肝细胞损伤促进丙型肝炎病毒转基因小鼠的肝肿瘤发生。
Cancer Sci. 2003 Aug;94(8):679-85. doi: 10.1111/j.1349-7006.2003.tb01502.x.
5
Hepatocarcinogenesis in hepatitis viral infection: lessons from transgenic mouse studies.病毒性肝炎感染中的肝癌发生：转基因小鼠研究的经验教训。
J Gastroenterol. 2002;37 Suppl 13:55-64. doi: 10.1007/BF02990101.
6
Virus-specific cofactor requirement and chimeric hepatitis C virus/GB virus B nonstructural protein 3.病毒特异性辅助因子需求与嵌合丙型肝炎病毒/GB病毒B非结构蛋白3
J Virol. 2000 May;74(9):4291-301. doi: 10.1128/jvi.74.9.4291-4301.2000.
本文引用的文献
1
Analysis of the cell tropism of HCV by using in vitro HCV-infected human lymphocytes and hepatocytes.利用体外感染丙型肝炎病毒（HCV）的人淋巴细胞和肝细胞分析HCV的细胞嗜性。
J Hepatol. 1997 Sep;27(3):445-54. doi: 10.1016/s0168-8278(97)80347-9.
2
Transfection of HepG2 cells with infectious hepatitis C virus genome.用丙型肝炎病毒感染性基因组转染HepG2细胞。
Am J Pathol. 1997 Aug;151(2):363-73.
3
Hepatitis C virus core protein induces hepatic steatosis in transgenic mice.丙型肝炎病毒核心蛋白在转基因小鼠中诱导肝脂肪变性。
J Gen Virol. 1997 Jul;78 ( Pt 7):1527-31. doi: 10.1099/0022-1317-78-7-1527.
4
Ultrastructural observations of viral particles within hepatitis C virus-infected human B lymphoblastoid cell line.丙型肝炎病毒感染的人B淋巴母细胞系内病毒颗粒的超微结构观察
Res Virol. 1997 Mar-Apr;148(2):153-9. doi: 10.1016/s0923-2516(97)89902-5.
5
Transgenic expression of hepatitis C virus structural proteins in the mouse.丙型肝炎病毒结构蛋白在小鼠中的转基因表达。
Hepatology. 1997 Apr;25(4):1014-21. doi: 10.1002/hep.510250437.
6
Hepatitis C virus core and E2 protein expression in transgenic mice.
Hepatology. 1997 Mar;25(3):719-27. doi: 10.1002/hep.510250338.
7
Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets.丙型肝炎病毒核心蛋白呈胞质定位，并与细胞脂质储存小滴相关联。
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1200-5. doi: 10.1073/pnas.94.4.1200.
8
Genetic analysis of the hepatitis C virus (HCV) genome from HCV-infected human T cells.
J Gen Virol. 1997 Feb;78 ( Pt 2):329-36. doi: 10.1099/0022-1317-78-2-329.
9
Sialadenitis histologically resembling Sjogren syndrome in mice transgenic for hepatitis C virus envelope genes.在丙型肝炎病毒包膜基因转基因小鼠中，组织学上类似于干燥综合征的涎腺炎。
Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):233-6. doi: 10.1073/pnas.94.1.233.
10
Regulated processing of hepatitis C virus core protein is linked to subcellular localization.丙型肝炎病毒核心蛋白的调控加工与亚细胞定位有关。
J Virol. 1997 Jan;71(1):657-62. doi: 10.1128/JVI.71.1.657-662.1997.
Zotero 插件