Serafino A, Valli M B, Alessandrini A, Ponzetto A, Carloni G, Bertolini L
Istituto di Medicina Sperimentale, Roma, Italy.
Res Virol. 1997 Mar-Apr;148(2):153-9. doi: 10.1016/s0923-2516(97)89902-5.
Hepatitis C virus (HCV)-infected TOFE cells, a human bone marrow-derived B-cell line, were studied by transmission electron microscopy (TEM). We reported the presence of virus-like particles, (VLPs) having a diameter of 45 nm on average, within the cytoplasm of HCV-infected cells and their absence in uninfected cells. The VLPs were mostly seen in dilated cisternae of endoplasmic reticulum (ER) and consisted of an electron-dense core and a membrane envelope with surface projections. In the HCV-infected TOFE cells, examination by TEM revealed tubular structures similar to those observed in liver cells of chimpanzees and humans infected by the virus. In some instances, the outer membrane of the particles was connected to the ER membrane, indicating possible virus budding into the cisternae. Rarely, coated vesicles containing a particle were seen. In most of the infected cells, enlarged cytoplasmic vacuoles filled with degenerative amorphous material were observed. The data suggest a flavivirus-like pattern of HCV morphogenesis in infected non-hepatic cells and identify lymphoblastoid TOFE cells as a valuable system for the study of the sequential steps of the infection process and the morphological effects produced by HCV infection.
利用透射电子显微镜（TEM）对丙型肝炎病毒（HCV）感染的TOFE细胞（一种人骨髓来源的B细胞系）进行了研究。我们报告在HCV感染细胞的细胞质中存在平均直径为45nm的病毒样颗粒（VLP），而在未感染细胞中则不存在。VLP大多见于内质网（ER）的扩张池，由电子致密核心和带有表面突起的膜包膜组成。在HCV感染的TOFE细胞中，TEM检查发现了与感染该病毒的黑猩猩和人类肝细胞中观察到的类似管状结构。在某些情况下，颗粒的外膜与ER膜相连，表明病毒可能出芽进入池内。很少见到含有颗粒的被膜小泡。在大多数感染细胞中，观察到充满退化性无定形物质的扩大细胞质空泡。这些数据表明HCV在感染的非肝细胞中具有黄病毒样形态发生模式，并确定淋巴母细胞样TOFE细胞是研究感染过程的连续步骤和HCV感染产生的形态学效应的有价值系统。
