Silva V, Fischman O, de Camargo Z P
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), Brasil.
Mycopathologia. 1997;139(2):79-85. doi: 10.1023/a:1006811704850.
Humoral immune responses against exoantigen components of oval, elliptic and round yeast forms of Malassezia furfur were analysed by ELISA and Western blotting assays, using sera from patients with pityriasis versicolor (PV), seborrheic dermatitis (SD) and healthy adults (HA), as control. Sera from patients with SD showed IgG anti-oval M. furfur titers ranging from 1/400 to 1/6400 showing geometric mean (GM) of 1/1472, higher than those obtained with sera from patients with PV (1/200 to 1/6400, GM = 1/1239). Both patient groups showed mean titres statistically superior (P < 0.05) than those obtained form HA (GM = 1/229). Similar data were also obtained with the elliptic and round antigens. However, the anti-oval IgG mean titers from patients' sera were much higher than those obtained with elliptic or round antigenic components (p < 0.05) Anti-M furfur IgM titers obtained from patient's sera with PV against all three exoantigens were statistically superior (p < 0.05) than HA group. Patients with SD showed IgM titers statistically superior (p < 0.05) only to oval yeasts of M. furfur. The IgA mean titers from patients' groups against the different morphological antigens were shown be slightly higher than those HA group. By Western blot, using rabbit anti-sera, the different antigenic components of M.furfur showed a close relationship mainly between oval and elliptic yeast cells antigens. The 70 kDa component of the M. furfur exoantigen of oval morphology was recognized by 84% of the PV patients' sera. On the other hand, SD patients' sera recognized 3 principal components of 70 kDa (100%), 65 kDa (67%) and 84 kDa (53%). These components may be considered immunological markers for PV and SD. Twenty-five percent of HA sera recognized the components of 65, 70 and 94 kDa. This investigation shows that M. furfur antigens can sensitize the host, mainly the oval yeast form of M. furfur with a very important specific IgG response in patients with SD and PV.
采用酶联免疫吸附测定（ELISA）和蛋白质印迹法，以花斑癣（PV）患者、脂溢性皮炎（SD）患者和健康成年人（HA）的血清为对照，分析了针对糠秕马拉色菌卵圆形、椭圆形和圆形酵母形式的外抗原成分的体液免疫反应。SD患者血清中抗卵圆形糠秕马拉色菌IgG滴度范围为1/400至1/6400，几何平均值（GM）为1/1472，高于PV患者血清（1/200至1/6400，GM = 1/1239）。两组患者的平均滴度在统计学上均高于HA组（GM = 1/229，P < 0.05）。椭圆形和圆形抗原也获得了类似的数据。然而，患者血清中的抗卵圆形IgG平均滴度远高于椭圆形或圆形抗原成分（p < 0.05）。PV患者血清中抗糠秕马拉色菌IgM滴度针对所有三种外抗原在统计学上均高于HA组（p < 0.05）。SD患者的IgM滴度仅在统计学上高于糠秕马拉色菌的卵圆形酵母（p < 0.05）。患者组针对不同形态抗原的IgA平均滴度略高于HA组。通过蛋白质印迹法，使用兔抗血清，糠秕马拉色菌的不同抗原成分主要显示出卵圆形和椭圆形酵母细胞抗原之间的密切关系。84%的PV患者血清识别出卵圆形形态的糠秕马拉色菌外抗原的70 kDa成分。另一方面，SD患者血清识别出70 kDa（100%）、65 kDa（67%）和84 kDa（53%）的3种主要成分。这些成分可被视为PV和SD的免疫标志物。25%的HA血清识别出65、70和94 kDa的成分。本研究表明，糠秕马拉色菌抗原可使宿主致敏，主要是糠秕马拉色菌的卵圆形酵母形式，在SD和PV患者中引发非常重要的特异性IgG反应。
