Hupp J G, Trope M, Mesaros S V, Aukhil I
Department of Endodontics, School of Dentistry, University of North Carolina at Chapel Hill 27599-7450, USA.
Endod Dent Traumatol. 1997 Oct;13(5):223-7. doi: 10.1111/j.1600-9657.1997.tb00044.x.
The purpose of this study was to evaluate a new storage solution, Conditioned Medium, vs Hank's balanced salt solution and ViaSpan with respect to the viability of periodontal ligament cells of exarticulated teeth. Teeth were stored for periods of 6, 48, and 96 hours in Hank's balanced salt solution, ViaSpan (Dupont Pharmaceuticals), or Conditioned Medium. Teeth were cultured for 24 hours in Dulbecco's medium supplemented with tritiated thymidine. The cultured teeth were sectioned and evaluated with autoradiography. Control teeth were extracted and immediately treated as above without storage. Mitotic activity was indicated by clusters of five or more grains over the nuclei of fibroblasts in the remaining periodontal membrane. The ratio of labeled to unlabeled cells (labeling index) was calculated for each treatment group. When storage time was compared across all groups, 96 hours was significantly different from 6 and 48 hours (P < 0.001 and P < 0.012 respectively). Storage time of 6 hours was not significantly different from 48 hours (P > 0.10). After comparison of the nine experimental groups with the control group, only Hank's balanced salt solution at 96 hours was significantly different (P < 0.004).
本研究的目的是评估一种新的储存溶液——条件培养基，与汉克平衡盐溶液和ViaSpan相比，对离体牙齿牙周膜细胞活力的影响。牙齿分别在汉克平衡盐溶液、ViaSpan（杜邦制药公司）或条件培养基中储存6小时、48小时和96小时。将牙齿在补充有氚标记胸腺嘧啶核苷的杜尔贝科培养基中培养24小时。对培养后的牙齿进行切片并通过放射自显影进行评估。对照牙齿拔除后立即按上述方法处理，不进行储存。有丝分裂活性通过剩余牙周膜中成纤维细胞核上五个或更多银粒的簇来表示。计算每个治疗组中标记细胞与未标记细胞的比例（标记指数）。当比较所有组的储存时间时，96小时与6小时和48小时有显著差异（分别为P < 0.001和P < 0.012）。6小时的储存时间与48小时无显著差异（P > 0.10）。将九个实验组与对照组进行比较后，只有96小时的汉克平衡盐溶液有显著差异（P < 0.004）。
