Hupp J G, Mesaros S V, Aukhil I, Trope M
Department of Endodontics, School of Dentistry, University of North Carolina at Chapel Hill, USA.
Endod Dent Traumatol. 1998 Apr;14(2):79-83. doi: 10.1111/j.1600-9657.1998.tb00815.x.
A previous study evaluated the viability of dog periodontal ligament cells as indicated by tritiated thymidine uptake after extended storage in Hank's balanced salt solution and Conditioned Medium. The purpose of this study was to evaluate histologic healing following the identical storage parameters established in the earlier study. Additionally, for Conditioned Medium, matched pairs (teeth evaluated for tritiated thymidine uptake and transplanted teeth) were examined in an attempt to correlate periodontal ligament vitality and healing. Forty-six extracted endodontically treated dogs' teeth were randomly grouped and stored in Hank's balanced salt solution or Conditioned Medium for 6, 48, and 96 h and then transplanted into 6-, 48-, and 96-h sockets. The control group teeth were transplanted without storage into 6-, 48-, or 96-h sockets. After 6 months the dogs were killed and the teeth were prepared for histologic evaluation according to Andreasen. Complete healing, inflammatory root resorption, and replacement resorption were evaluated and compared. Overall, significantly better healing was observed for teeth stored in Conditioned Medium than for teeth stored in Hank's balanced salt solution. Conditioned Medium was not significantly different from controls. Additionally, there was a positive correlation between periodontal ligament viability and healing for Conditioned Medium. These results confirmed the importance of periodontal ligament viability in successful replantation and the potential of Conditioned Medium as a storage medium for avulsed teeth.
先前的一项研究评估了犬牙周膜细胞在汉克平衡盐溶液和条件培养基中长时间储存后，通过氚标记胸腺嘧啶核苷摄取所显示的活力。本研究的目的是评估在早期研究中确定的相同储存参数后的组织学愈合情况。此外，对于条件培养基，对配对组（评估氚标记胸腺嘧啶核苷摄取的牙齿和移植牙齿）进行了检查，以试图关联牙周膜活力和愈合情况。将46颗经牙髓治疗的拔除犬牙随机分组，在汉克平衡盐溶液或条件培养基中储存6、48和96小时，然后移植到6、48和96小时的牙槽窝中。对照组牙齿不储存直接移植到6、48或96小时的牙槽窝中。6个月后处死犬只，并根据安德烈亚森方法对牙齿进行组织学评估。评估并比较完全愈合、炎性牙根吸收和替代性吸收情况。总体而言，观察到储存在条件培养基中的牙齿愈合情况明显优于储存在汉克平衡盐溶液中的牙齿。条件培养基与对照组无显著差异。此外，对于条件培养基，牙周膜活力与愈合之间存在正相关。这些结果证实了牙周膜活力在成功再植中的重要性以及条件培养基作为脱位牙储存介质的潜力。
