Acta Stomatol Croat. 2015 Mar;49(1):21-6. doi: 10.15644/asc49/1/3.
The aim of the present in vitro study was to investigate the potential of a storage medium, probiotic yogurt (Bifidibacterium animalis DN 173010) in comparison with Hank's balanced salt solution (HBSS), saline and milk in maintaining viable periodontal ligament (PDL) cells on simulated avulsed teeth.
Thirty-six freshly extracted single-rooted human teeth with closed apices were divided into six experimental groups (N=6). The teeth were extracted as atraumatically as possible and washed in sterile saline solution to eliminate residual blood. Following extractions, the coronal 3 mm of PDL tissues were scraped with a #15 scalpel to remove cells that may have been damaged. The positive and negative controls corresponded to 0 minutes and an 8-hour dry time, respectively. After extraction, the positive control teeth were immediately treated with dispase and collagenase. The negative control teeth were bench-dried for 8 h, with no follow-up storage solution time, and then placed in the dispase and collagenase. The number of viable protective least significant difference PDL cells were counted under a light microscope with a hemocytometer at 20× magnification and analyzed. Statistical analysis of the data was accomplished using Nonparametric ANOVA complemented by Kruskal-Wallis Test and Dunn's Multiple Comparisons Test.
Positive control was found to be significantly better than the others, there were statistically significant differences between positive control and other test groups (p=0.000). The teeth stored in positive control demonstrated the highest number of viable PDL cells followed in order by probiotic yogurt, HBSS, saline and milk.
Bifidibacterium animalis DN 173010 seems to be an alternative for the temporary storage of avulsed teeth, due to high number of viable PDL cells. Probiotics may be suitable transport media for avulsed teeth, but further research is warranted using the commercially available products.
本体外研究的目的是研究一种储存介质——益生菌酸奶（动物双歧杆菌DN 173010）与汉克平衡盐溶液（HBSS）、生理盐水和牛奶相比，在维持模拟脱位牙上牙周膜（PDL）细胞活力方面的潜力。
将36颗新鲜拔除的单根闭合根尖恒牙分为6个实验组（N = 6）。尽可能无创地拔除牙齿，并用无菌盐溶液冲洗以清除残留血液。拔牙后，用15号手术刀刮除冠方3 mm的PDL组织，以去除可能受损的细胞。阳性和阴性对照分别对应0分钟和8小时干燥时间。拔牙后，阳性对照牙立即用分散酶和胶原酶处理。阴性对照牙在实验台上干燥8小时，不使用后续储存溶液，然后置于分散酶和胶原酶中。在20倍放大倍数下，用血细胞计数器在光学显微镜下计数存活的PDL细胞数量并进行分析。数据的统计分析采用非参数方差分析，并辅以Kruskal-Wallis检验和Dunn多重比较检验。
发现阳性对照明显优于其他组，阳性对照与其他测试组之间存在统计学显著差异（p = 0.000）。储存在阳性对照中的牙齿显示出存活的PDL细胞数量最多，其次依次是益生菌酸奶、HBSS、生理盐水和牛奶。
由于存活的PDL细胞数量较多，动物双歧杆菌DN 173010似乎是脱位牙临时储存的一种替代方法。益生菌可能是脱位牙合适的运输介质，但使用市售产品进行进一步研究是有必要的。
