Hachicha M, Rathanaswami P, Naccache P H, McColl S R
Department of Microbiology and Immunology, University of Adelaide, South Australia, Australia.
J Immunol. 1998 Jan 1;160(1):449-54.
Production of chemokines (chemotactic cytokines) by neutrophils is likely to be important in the regulation of inflammation and the control of infection. In this study we show that exposure of human neutrophils to various microbial pathogens leads to the production of both macrophage inflammatory protein 1alpha (MIP-1alpha) and IL-8. The bacterial microbes, Salmonella typhimurium and Pseudomonas aeruginosa, and Staphylococcus aureus all strongly induced both IL-8 and MIP-1alpha secretion, whereas Streptococcus pneumoniae, Staphylococcus epidermidis, and the opportunistic yeast Candida albicans were less potent. Saccharomyces cerevisiae and zymosan both induced IL-8 secretion but failed to stimulate that of MIP-1alpha. Coincubation of neutrophils with the proinflammatory cytokine TNF-alpha and the micro-organisms also led to differential expression of MIP-1alpha and IL-8. Significant enhancement of the induction of both MIP-1alpha and IL-8 by S. typhimurium, P. aeruginosa, and S. pneumoniae as well as by C. albicans was observed. In contrast, while IL-8 production in response to S. cerevisiae and zymosan was enhanced in the presence of TNF-alpha, no MIP-1alpha was produced. These combined results indicate that while neutrophils exposed to some micro-organisms alone or in the presence of inflammatory cytokines such as TNF-alpha will produce both MIP-1alpha and IL-8, resulting in generation of signals for the recruitment of mononuclear leukocytes and neutrophils, respectively, certain types of microorganisms can skew this response toward synthesis of IL-8.
中性粒细胞产生趋化因子（趋化性细胞因子）可能在炎症调节和感染控制中起重要作用。在本研究中，我们发现人类中性粒细胞暴露于各种微生物病原体后会产生巨噬细胞炎性蛋白1α（MIP-1α）和白细胞介素8（IL-8）。细菌微生物鼠伤寒沙门氏菌、铜绿假单胞菌和金黄色葡萄球菌均强烈诱导IL-8和MIP-1α的分泌，而肺炎链球菌、表皮葡萄球菌和机会性酵母白色念珠菌的诱导作用较弱。酿酒酵母和酵母聚糖均诱导IL-8分泌，但未能刺激MIP-1α的分泌。中性粒细胞与促炎细胞因子肿瘤坏死因子-α（TNF-α）和微生物共同孵育也导致MIP-1α和IL-8的差异表达。观察到鼠伤寒沙门氏菌、铜绿假单胞菌、肺炎链球菌以及白色念珠菌对MIP-1α和IL-8的诱导作用均显著增强。相比之下，虽然在TNF-α存在的情况下，酿酒酵母和酵母聚糖诱导的IL-8产生增加，但未产生MIP-1α。这些综合结果表明，虽然单独暴露于某些微生物或在炎性细胞因子如TNF-α存在的情况下，中性粒细胞会产生MIP-1α和IL-8，分别导致单核白细胞和中性粒细胞募集信号的产生，但某些类型的微生物可使这种反应偏向于IL-8的合成。
