Björkman U, Ekholm R, Ericson L E, Ofverholm T
Mol Cell Endocrinol. 1976 Jun-Jul;5(1-2):3-17. doi: 10.1016/0303-7207(76)90065-4.
The purpose of this study was to explore the nature of the protein(s) in the exocytotic vesicles in the thyroid follicle cells and to ascertain whether or not thyroglobulin and peroxidase are transported by the same vesicles through the apical region of the cells to the follicle lumen. The study was performed on rats pretreated with thyroxine for 2 days in order to inhibit endocytosis. A fraction of exocytotic vesicles was isolated by centrifugation in continuous and discontinuous sucrose density gradients. The protein content of the vesicles were analysed by electrophoresis in continuous polyacrylamide gradient gels. The vesicles contained (uniodinated) thyroglobulin, 12-S protein and thyralbumin. Parallel histochemical studies in the electron microscope. These observations have important bearings on the mechanisms for thyroglobulin iodination, since it has been demonstrated that iodination does not occur in the exocytotic vesicles but in connection with the opening of the vesicles at the apical cell surface.
本研究的目的是探索甲状腺滤泡细胞中胞吐小泡内蛋白质的性质，并确定甲状腺球蛋白和过氧化物酶是否通过相同的小泡经细胞顶端区域转运至滤泡腔。本研究在经甲状腺素预处理2天以抑制内吞作用的大鼠身上进行。通过在连续和不连续蔗糖密度梯度中离心分离出一部分胞吐小泡。通过在连续聚丙烯酰胺梯度凝胶中进行电泳分析小泡的蛋白质含量。这些小泡含有（未碘化的）甲状腺球蛋白、12 - S蛋白和甲状腺白蛋白。在电子显微镜下进行平行组织化学研究。这些观察结果对甲状腺球蛋白碘化机制具有重要意义，因为已证明碘化并非发生在胞吐小泡中，而是与小泡在细胞顶端表面的开口有关。
