Katz D, Kohn A
Dev Biol Stand. 1976;33:290-6.
In mammals the antibody activity in secretions is associated with the secretory IgA. This unique immunoglobulin plays a major role in immunity against infectious diseases of the alimentary and the respiratory tracts. We found a similar system of local immunity against Newcastle disease virus (NDV) in the fowl. Airway washings and bile globulins of chickens were examined by immunodiffusion and immunoelectrophoretic methods. No antigenic differences were found between the serum IgA and the secretory IgA of airway washings and of bile. The chicken IgA differs from mammalian IgA by lacking secretory component. Antibodies in the airway washings and in tears of chickens immunized by aerosol ir i.m. NDV vaccine (attenuated or inactive) were compared to serum antibodies produced by similar methods of administration. HI antibodies in secretions reached higher levels after aerosol vaccination than after i.m. administration, whereas in serum the situation was reversed. The antibody activity in airway washings and in tears is associated with IgA, while in serum the main antibody is IgG.
在哺乳动物中，分泌物中的抗体活性与分泌型IgA相关。这种独特的免疫球蛋白在抵抗消化道和呼吸道传染病的免疫中起主要作用。我们在禽类中发现了类似的针对新城疫病毒（NDV）的局部免疫体系。采用免疫扩散和免疫电泳方法检测了鸡的气道冲洗液和胆汁球蛋白。气道冲洗液和胆汁的血清IgA与分泌型IgA之间未发现抗原差异。鸡的IgA与哺乳动物的IgA不同，缺乏分泌成分。将经气溶胶或肌肉注射新城疫病毒疫苗（减毒或灭活）免疫的鸡的气道冲洗液和泪液中的抗体与通过类似给药方法产生的血清抗体进行了比较。气溶胶疫苗接种后，分泌物中的血凝抑制（HI）抗体水平高于肌肉注射后，而血清中的情况则相反。气道冲洗液和泪液中的抗体活性与IgA相关，而血清中的主要抗体是IgG。
