Fischer M B, Goerg S, Shen L, Prodeus A P, Goodnow C C, Kelsoe G, Carroll M C
Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
Science. 1998 Apr 24;280(5363):582-5. doi: 10.1126/science.280.5363.582.
Affinity-driven selection of B lymphocytes within germinal centers is critical for the development of high-affinity memory cells and host protection. To investigate the role of the CD21/CD35 coreceptor in B cell competition for follicular retention and survival within the germinal center, either Cr2+ or Cr2null lysozyme-specific transgenic B cells were adoptively transferred into normal mice immunized with duck (DEL) or turkey (TEL) lysozyme, which bind with different affinities. In mice injected with high-affinity turkey lysozyme, Cr2null B cells responded by follicular retention; however, they could not survive within germinal centers. This suggests that CD21 provides a signal independent of antigen that is required for survival of B cells in the germinal center.
生发中心内B淋巴细胞的亲和力驱动选择对于高亲和力记忆细胞的发育和宿主保护至关重要。为了研究CD21/CD35共受体在生发中心内B细胞竞争滤泡滞留和存活中的作用，将Cr2+或Cr2基因敲除的溶菌酶特异性转基因B细胞过继转移到用鸭（DEL）或火鸡（TEL）溶菌酶免疫的正常小鼠体内，这两种溶菌酶具有不同的亲和力。在用高亲和力火鸡溶菌酶注射的小鼠中，Cr2基因敲除的B细胞通过滤泡滞留作出反应；然而，它们无法在生发中心内存活。这表明CD21提供了一个独立于抗原的信号，这是生发中心内B细胞存活所必需的。
