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高多重蛋白和全转录组共定位与空间 CITE-seq 在细胞分辨率下。

High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq.

机构信息

Department of Biomedical Engineering, Yale University, New Haven, CT, USA.

Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT, USA.

出版信息

Nat Biotechnol. 2023 Oct;41(10):1405-1409. doi: 10.1038/s41587-023-01676-0. Epub 2023 Feb 23.

DOI:10.1038/s41587-023-01676-0
PMID:36823353
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10567548/
Abstract

In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further applied the method to measure 273 proteins and transcriptome in human tissues, revealing spatially distinct germinal center reactions in tonsil and early immune activation in skin at the Coronavirus Disease 2019 mRNA vaccine injection site.

摘要

在这项研究中，我们将转录组和表位的共索引（CITE）扩展到空间维度，并展示了高通量蛋白质和全转录组的共映射。我们使用空间 CITE 测序对多种小鼠组织类型中的 189 种蛋白质和全转录组进行了分析，然后进一步将该方法应用于测量人组织中的 273 种蛋白质和转录组，揭示了在冠状病毒疾病 2019 mRNA 疫苗注射部位的扁桃体中空间上不同的生发中心反应和皮肤中的早期免疫激活。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/09be09ec7a24/41587_2023_1676_Fig9_ESM.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/47ba16fe3c35/41587_2023_1676_Fig6_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/46507224122c/41587_2023_1676_Fig7_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/fb86b5a314d5/41587_2023_1676_Fig8_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/09be09ec7a24/41587_2023_1676_Fig9_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/86b964e27f62/41587_2023_1676_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/538c12e76882/41587_2023_1676_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/49b75aa2f013/41587_2023_1676_Fig3_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/196fdcd5e89b/41587_2023_1676_Fig4_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/c30d7de7e415/41587_2023_1676_Fig5_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/47ba16fe3c35/41587_2023_1676_Fig6_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/46507224122c/41587_2023_1676_Fig7_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/fb86b5a314d5/41587_2023_1676_Fig8_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd28/10567548/09be09ec7a24/41587_2023_1676_Fig9_ESM.jpg

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