Croix D A, Ahearn J M, Rosengard A M, Han S, Kelsoe G, Ma M, Carroll M C
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
J Exp Med. 1996 Apr 1;183(4):1857-64. doi: 10.1084/jem.183.4.1857.
Several lines of evidence indicate that antibody responses to T-dependent antigens require complement receptors expressed on either B lymphocytes or follicular dendritic cells. We have used RAG-2 deficient blastocyst complementation to create mice specifically lacking B cell complement receptors. Despite normal expression of complement receptor 1 (CR1[CD35]) and CR2 (CD21) on follicular dendritic cells, these mice have a profound defect in their capacity to mount a T-dependent antibody response. This is the first direct demonstration in vivo that B cell expression of complement receptors is required for a humoral immune response. This is the first direct demonstration in vivo that B cell expression of complement receptors is required for a humoral immune response. This suggests that CD21 and/or CD35 on B lymphocytes may be required for cellular activation, adsorptive endocytosis of antigen, recruitment to germinal centers, and/or protection from apoptosis during the humoral response to T-dependent antigens.
多条证据表明，针对T细胞依赖性抗原的抗体反应需要B淋巴细胞或滤泡树突状细胞上表达的补体受体。我们利用RAG-2缺陷型囊胚互补技术培育出了特异性缺乏B细胞补体受体的小鼠。尽管滤泡树突状细胞上的补体受体1（CR1[CD35]）和CR2（CD21）表达正常，但这些小鼠在产生T细胞依赖性抗体反应的能力上存在严重缺陷。这是首次在体内直接证明体液免疫反应需要B细胞表达补体受体。这表明，在对T细胞依赖性抗原的体液反应过程中，B淋巴细胞上的CD21和/或CD35可能是细胞活化、抗原吸附性内吞作用、募集到生发中心和/或防止细胞凋亡所必需的。
