Johnsson H, Heldin P, Sedin G, Laurent T C
Department of Pediatrics, Uppsala University, Sweden.
Ups J Med Sci. 1997;102(3):199-209. doi: 10.3109/03009739709178941.
Fetal human lung fibroblasts and feline lung epithelial cells were exposed to either a surfactant or N-acetylcysteine in various concentrations for 24-48 hours, after which the hyaluronan concentration in the culture medium was determined. Most of the experiments showed no stimulatory effect of either artificial or natural surfactant on hyaluronan synthesis. N-acetylcysteine 5-100 mg/mL induced progressive stimulation of hyaluronan synthesis by human fetal lung fibroblasts, resulting in a maximum hyaluronan concentration six times that released by unexposed cells. A slight increase in hyaluronan synthesis was also observed after exposure of feline fetal lung epithelial cells to N-acetylcysteine 50-100 micrograms/mL.
将人胎儿肺成纤维细胞和猫肺上皮细胞暴露于不同浓度的表面活性剂或N-乙酰半胱氨酸中24至48小时，之后测定培养基中的透明质酸浓度。大多数实验表明，人工或天然表面活性剂对透明质酸合成均无刺激作用。5至100毫克/毫升的N-乙酰半胱氨酸可诱导人胎儿肺成纤维细胞对透明质酸合成的逐渐刺激，导致透明质酸的最大浓度是未暴露细胞释放浓度的六倍。在猫胎儿肺上皮细胞暴露于50至100微克/毫升的N-乙酰半胱氨酸后，也观察到透明质酸合成略有增加。
