Bazan H A, Alkhatib G, Broder C C, Berger E A
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0445, USA.
J Virol. 1998 May;72(5):4485-91. doi: 10.1128/JVI.72.5.4485-4491.1998.
Coreceptor usage by Envs from diverse primary human immunodeficiency virus type 1 isolates was analyzed by a vaccinia virus-based expression and assay system. Usage of recombinant CCR5 and CXCR4 correlated closely with fusogenicity toward macrophages and T-cell lines expressing endogenous coreceptors. Surprisingly, recombinant CCR3 was utilized by most primary and T-cell-line-adapted Envs. Endogenous CXCR4 in macrophages was functional as a coreceptor.
利用基于痘苗病毒的表达和检测系统，分析了来自多种原发性人类免疫缺陷病毒1型分离株的包膜蛋白（Env）对共受体的使用情况。重组CCR5和CXCR4的使用与对表达内源性共受体的巨噬细胞和T细胞系的融合活性密切相关。令人惊讶的是，大多数原发性和适应T细胞系的Env利用重组CCR3。巨噬细胞中的内源性CXCR4作为共受体发挥功能。
