Nguyen H H, Boyaka P N, Moldoveanu Z, Novak M J, Kiyono H, McGhee J R, Mestecky J
Department of Microbiology and The Immunobiology Vaccine Center, University of Alabama at Birmingham, 35294-2170, USA.
J Virol. 1998 May;72(5):4534-6. doi: 10.1128/JVI.72.5.4534-4536.1998.
We have investigated the mechanisms involved in the clearance of viral infection at the epithelium level by analyzing the activity of influenza virus-specific cytotoxic T lymphocytes (CTL) against virus-infected CMT-93 intestinal epithelial cells. Epithelial cells infected with live influenza virus effectively present viral antigens and were lysed by both homotypic and heterotypic influenza virus-specific CD8+ T cells. These results shed new light on the control of viral infection through the elimination of virus-infected epithelial cells by virus-specific CTL and demonstrate that CMT-93 cells furnish an appropriate model for in vitro evaluation of CTL activity against virus-infected epithelial cells.
我们通过分析流感病毒特异性细胞毒性T淋巴细胞(CTL)对病毒感染的CMT-93肠上皮细胞的活性,研究了上皮水平清除病毒感染所涉及的机制。感染活流感病毒的上皮细胞能有效呈递病毒抗原,并被同型和异型流感病毒特异性CD8 + T细胞裂解。这些结果为通过病毒特异性CTL消除病毒感染的上皮细胞来控制病毒感染提供了新的思路,并证明CMT-93细胞为体外评估CTL对病毒感染上皮细胞的活性提供了一个合适的模型。