Bennink J R, Yewdell J W, Smith G L, Moss B
J Virol. 1987 Apr;61(4):1098-102. doi: 10.1128/JVI.61.4.1098-1102.1987.
It has recently been shown that antiviral major histocompatibility complex class I-restricted cytotoxic T lymphocytes can recognize proteins that serve as internal viral structural components (influenza A virus nucleoprotein, vesicular stomatitis virus nucleocapsid protein). To further examine the role of internal viral proteins in cytotoxic T-lymphocyte recognition, we constructed recombinant vaccinia viruses containing individual influenza A virus genes encoding three viral polymerases (PB1, PB2, PA) and a protein not incorporated into virions (NS1). We found that cells infected with each of these recombinant vaccinia viruses could be lysed by anti-influenza cytotoxic T lymphocytes. Cytotoxic T-lymphocyte responsiveness to the individual viral antigens varied greatly between mouse strains. By using congenic mouse strains, responsiveness to PB1 and PB2 was found to cosegregate with major histocompatibility complex haplotype. These findings provide further evidence that internal antigens play a critical role in cytotoxic T-lymphocyte recognition of virus-infected cells. Additionally, they suggest that the cytotoxic T-lymphocyte response to viral antigens may often be restricted to only a fraction of the major histocompatibility complex class I repertoire.
最近研究表明，抗病毒的主要组织相容性复合体I类限制性细胞毒性T淋巴细胞能够识别作为病毒内部结构成分的蛋白质（甲型流感病毒核蛋白、水疱性口炎病毒核衣壳蛋白）。为了进一步研究病毒内部蛋白在细胞毒性T淋巴细胞识别中的作用，我们构建了重组痘苗病毒，其包含编码三种病毒聚合酶（PB1、PB2、PA）和一种未掺入病毒粒子的蛋白（NS1）的单个甲型流感病毒基因。我们发现，感染这些重组痘苗病毒中的每一种的细胞都可被抗流感细胞毒性T淋巴细胞裂解。细胞毒性T淋巴细胞对各个病毒抗原的反应性在小鼠品系之间差异很大。通过使用同源近交系小鼠品系，发现对PB1和PB2的反应性与主要组织相容性复合体单倍型共分离。这些发现提供了进一步的证据，表明内部抗原在细胞毒性T淋巴细胞识别病毒感染细胞中起关键作用。此外，它们表明细胞毒性T淋巴细胞对病毒抗原的反应可能常常仅局限于主要组织相容性复合体I类库的一部分。
