Blanchard A, Ferris S, Chamaret S, Guétard D, Montagnier L
Unité d'Oncologie Virale, Institut Pasteur, Paris, France.
J Virol. 1998 May;72(5):4537-40. doi: 10.1128/JVI.72.5.4537-4540.1998.
We have investigated the molecular evidence in favor of the transmission of human immunodeficiency virus (HIV) from an HIV-infected surgeon to one of his patients. After PCR amplification, the env and gag sequences from the viral genome were cloned and sequenced. Phylogenetic analysis revealed that the viral sequences derived from the surgeon and his patient are closely related, which strongly suggests that nosocomial transmission occurred. In addition, these viral sequences belong to group M of HIV type 1 but are divergent from the reference sequences of the known subtypes.
我们调查了支持人类免疫缺陷病毒(HIV)从一名感染HIV的外科医生传播给他的一名患者的分子证据。经过聚合酶链反应(PCR)扩增后,对病毒基因组中的env和gag序列进行了克隆和测序。系统发育分析表明,来自外科医生及其患者的病毒序列密切相关,这强烈提示发生了医院内传播。此外,这些病毒序列属于HIV-1的M组,但与已知亚型的参考序列不同。